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Spinal Cord Transcriptome Analysis Using Suppression Subtractive Hybridization and Mirror Orientation Selection

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Summary

Comparison of cDNA libraries derived from the spinal cord with those derived from the visual cortex by means of forward and reverse subtractive hybridization resulted in the cataloguing of 60 genes differentially expressed in the spinal cord.

1. The differentially expressed genes represent a mixture of novel and known sequences with known and unknown protein products.

2. The possibility that the subtraction process was simply overwhelmed by background sequences was significantly reduced by several observations including comparisons between suppression subtractive hybridization (SSH) and mirror orientation selection (MOS).

3. Nearly half of all genes up-regulated in the spinal cord are of myelin origin.

4. Twenty-five percent of all up-regulated clones in the spinal cord versus the visual cortex are for proteolipid protein.

5. Ten percent of all up-regulated clones in spinal cord versus visual cortex are for ferretin heavy chain, which is known to be produced in oligodendroglial cells in the CNS.

6. Two of the up-regulated sequences, proteolipid protein and N-myc down-regulated gene 4, are identified with genes known to directly affect neuron survival.

7. Two of the up-regulated genes, ferritin and transferrin, are indirectly associated with apoptosis through their ability to sequester iron and reduce free radical formation.

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Acknowledgments

This work is supported by a bequest from the estate of Frances and Wesley Johnson and the generous donations of numerous individuals to Solomon Park Research Institute. We would like to thank the members of the board of directors of Solomon Park Research Institute for their tireless dedication to the Institute and the ongoing research effort.

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Correspondence to Patric A. Clapshaw.

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Lathia, K.B., Yan, Z. & Clapshaw, P.A. Spinal Cord Transcriptome Analysis Using Suppression Subtractive Hybridization and Mirror Orientation Selection. Cell Mol Neurobiol 26, 259–275 (2006). https://doi.org/10.1007/s10571-006-9052-x

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  • DOI: https://doi.org/10.1007/s10571-006-9052-x

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