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Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress

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Abstract

The radioprotective effect of geraniin, a tannin compound isolated from Nymphaea tetragona Georgi var. (Nymphaeaceae), against γ-radiation-induced damage was investigated in Chinese hamster lung fibroblast (V79-4) cells. Geraniin recovered cell viability detected by MTT test and colony formation assay, which was compromised by γ-radiation, and reduced the γ-radiation-induced apoptosis by the inhibition of loss of the mitochondrial membrane potential. Geraniin protected cellular components (lipid membrane, cellular protein, and DNA) damaged by γ-radiation, which was detected by lipid peroxidation, protein carbonyl formation, and comet assay. Geraniin significantly reduced the level of intracellular reactive oxygen species generated by γ-radiation, which was detected using spectrofluorometer, flow cytometer, and confocal microscope after 2′,7′-dichlorodihydrofluorescein diacetate staining. Geraniin normalized the superoxide dismutase and catalase activities, which were decreased by γ-radiation. These results suggest that geraniin protects cells against radiation-induced oxidative stress via enhancing of antioxidant enzyme activities and attenuating of cellular damage.

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Acknowledgements

This study was performed under the program of Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R&D Programs funded by the Ministry of Science & Technology (MOST) of Korea.

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Authors and Affiliations

  1. School of Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju-si, 690-756, South Korea

    Kyoung Ah Kang, Rui Zhang, Mei Jing Piao, Ki Cheon Kim & Jin Won Hyun

  2. Department of Microbiology and Cancer Research Institute, College of Medicine, Seoul National University, Seoul, 110-799, South Korea

    In Kyung Lee & Bum Joon Kim

  3. Department of Internal Medicine, School of Medicine, Chosun University, 375 Seosuk-dong, Gwangju, 501-759, South Korea

    Sang Young Kim

  4. Department of Veterinary Medicine, Jeju National University, Jeju-si, 690-756, South Korea

    Taekyun Shin

  5. Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju-si, 690-756, South Korea

    Nam Ho Lee

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  1. Kyoung Ah Kang
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  2. In Kyung Lee
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  3. Rui Zhang
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  10. Jin Won Hyun
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Correspondence to Jin Won Hyun.

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Kyoung Ah Kang and In Kyung Lee contributed equally to this study.

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Kang, K.A., Lee, I.K., Zhang, R. et al. Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress. Cell Biol Toxicol 27, 83–94 (2011). https://doi.org/10.1007/s10565-010-9172-4

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  • DOI: https://doi.org/10.1007/s10565-010-9172-4

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