Abstract
Deep second and third degree burns treatment requires fibroblasts, keratinocytes and other skin cells in order to grow new dermis and epidermis. Cells can proliferate, secrete growth factors and extracellular matrix required to repair the damaged tissue. Radiosterilized human amnion and radiosterilized pig skin have been used as natural origin skin dressings for burned patients. Adipose-derived mesenchymal stem cells can differentiate into fibroblasts and keratinocytes and improve wound-healing progress. These cells can stimulate vascular tissue formation, release growth factors, synthetize new extracellular matrix and immunoregulate other cells. In this study, we developed mesenchymal stem cells-cellularized skin substitutes based from radiosterilized human amnion or pig skin. Third-degree burns were induced in mice animal models to evaluate the effect of cellularized skin substitutes on burn wound healing. Mesenchymal phenotype was immunophenotypically confirmed by flow cytometry and cell viability was close to 100%. Skin recovery was evaluated in burned mice after seven and fourteen days post-coverage with cellularized and non-cellularized sustitutes. Histological techniques and immunofluorescence were used to evaluate re-epithelization and type I collagen deposition. We determined that cellularized-human amnion or cellularized-pig skin in combination with mesenchymal stem cells improve extracellular matrix deposition. Both cellularized constructs increase detection of type I collagen in newly formed mouse skin and can be potentially used as skin coverage for further clinical treatment of burned patients.
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Data availability statement
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors express their gratitude to Dr. Javier PÉREZ GALLAGA and Dr. Elena CONTRERAS FIGUEROA for surgical procedures and animal care. BSc. Julieta GARCÍA-LÓPEZ for histological processing at Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra. We also acknowledge Daniel REBOYO BARRIOS for biomaterial processing at the Banco de Tejidos Radioesterilizados del Instituto Nacional de Investigaciones Nucleares.
Funding
This work was supported by the Consejo Nacional de Ciencia y Tecnología, Project numbers 262103 and B-S-40505. It was also supported by the International Atomic Energy Agency through the IAEA Research Contract No. 18278 (2014–2019).
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B. Cabello-Arista and Y. Melgarejo-Ramírez have contributed equally to this work.
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Cabello-Arista, B., Melgarejo-Ramírez, Y., Retana-Flores, A. et al. Effects of mesenchymal stem cell culture on radio sterilized human amnion or radio sterilized pig skin in burn wound healing. Cell Tissue Bank 25, 255–267 (2024). https://doi.org/10.1007/s10561-021-09976-y
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DOI: https://doi.org/10.1007/s10561-021-09976-y