Abstract
Sperm cryopreservation is a routine method in andrology and IVF laboratory. However, the sperm quality and its fertilizing capacity have been decreased during this process. The purpose of this experiment was to determine the role of myoinositol as a supplement in amelioration of total and progressive sperm motility, DNA fragmentation, total antioxidant capacity (TAC), reactive oxygen species (ROS), and lipid peroxidation after the freezing–thawing process on patients with oligoasthenoteratozoospermia (OAT) syndrome. Semen samples obtained from 40 patients were divided into two aliquots and freezed with simple and 2 mg/mL myoinositol (MYO) supplemented freezing media. All samples were thawed and assessed after one month. Semen parameters were analyzed in terms of the motility by CASA, the level of total ROS by fluorimetry, TAC and MDA by colorimetric assay and finally DNA fragmentation by TUNEL assay. Our results clearly showed that MYO could improve total (37.46 vs. 12.91, p < 0.001) and progressive motility (21.92 vs. 6.49, p < 0.001) in experimental group compared to control group. A higher TAC level was observed in the MYO treated group in comparison to control group (1.11 vs. 0.91, p = 0.05). While MYO supplementation could not be effective on ROS level, it reduced DNA fragmentation of sperm after freeze–thaw process (p = 0.01). Therefore, MYO could be a good supplement for sperm freezing to reduce the detrimental effects of freezing process especially on DNA integrity, which is an important factor in the success of ART, in OAT suffered patients.
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References
Abad C, Amengual MJ, Gosalvez J, Coward K, Hannaoui N, Benet J, Prats J (2013) Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA. Andrologia 45(3):211–216. https://doi.org/10.1111/and.12003
Agarwal A, Sekhon LH (2011) Oxidative stress and antioxidants for idiopathic oligoasthenoteratospermia: is it justified? Indian J Urol IJU J Urol Soc India 27(1):74
Agarwal A, Tvrda E (2017) Slow freezing of human sperm. In: Cryopreservation of mammalian gametes and embryos. Springer, pp 67–78
Agarwal A, Nallella KP, Allamaneni SS, Said TM (2004) Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 8(6):616–627
Agarwal A, Makker K, Sharma R (2008) Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol 59(1):2–11
Agarwal A, Varghese AC, Sharma RK (2009) Markers of oxidative stress and sperm chromatin integrity. In: Molecular endocrinology. Springer, pp 377–402
Agarwal A, Mulgund A, Sharma R, Sabanegh E (2014) Mechanisms of oligozoospermia: an oxidative stress perspective. Syst Biol Reprod Med 60(4):206–216. https://doi.org/10.3109/19396368.2014.918675
Amaral A, Lourenço BR, Marques MN, Ramalho-Santos J (2013) Mitochondria functionality and sperm quality. Reproduction 146(5):R163–R174
Asadpour R, Jafari R, Tayefi-Nasrabadi H (2012) The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iran J Vet Res 13(3):246–249
Badade G, More K, Narshetty G, Badade VZ, Yadav B (2011) Human seminal oxidative stress: correlation with antioxidants and sperm quality parameters. Ann Biol Res 2(5):351–359
Barrera G (2012) Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncol 2012
Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M (2010) Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 60(2):235–237. https://doi.org/10.1016/j.cryobiol.2009.10.012
Brugnon F, Ouchchane L, Pons-Rejraji H, Artonne C, Farigoule M, Janny L (2013) Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Hum Reprod 28(8):2045–2057
Cao X-W, Lin K, Li C-Y, Yuan C-W (2011) A review of WHO laboratory manual for the examination and processing of human semen. Natl J Androl 17(12):1059–1063
Chian R-C, Quinn P (2010) Fertility cryopreservation. Cambridge University Press, Cambridge
Colagar AH, Karimi F, Jorsaraei SGA (2013) Correlation of sperm parameters with semen lipid peroxidation and total antioxidants levels in astheno-and oligoasheno-teratospermic men. Iran Red Crescent Med J 15(9):780
Colone M, Marelli G, Unfer V, Bozzuto G, Molinari A, Stringaro A (2010) Inositol activity in oligoasthenoteratospermia–an in vitro study. Eur Rev Med Pharmacol Sci 14(10):891–896
Condorelli R, La Vignera S, Di Bari F, Unfer V, Calogero A (2011) Effects of myoinositol on sperm mitochondrial function in-vitro. Eur Rev Med Pharmacol Sci 15(2):129–134
Condorelli RA, La Vignera S, Bellanca S, Vicari E, Calogero AE (2012) Myoinositol: does it improve sperm mitochondrial function and sperm motility? Urology 79(6):1290–1295
Condorelli R, La Vignera S, Mongioi L, Vitale S, Lagana A, Cimino L, Calogero A (2017) Myo-inositol as a male fertility molecule: speed them up. Eur Rev Med Pharmacol Sci 21(2 Suppl):30–35
Dinkova A, Martinov D, Konova E (2017) Efficacy of myo-inositol in the clinical management of patients with asthenozoospermia. Eur Rev Med Pharmacol Sci 21(2 Suppl):62–65
Donà G, Sabbadin C, Fiore C, Bragadin M, Giorgino FL, Ragazzi E, Armanini D (2012) Inositol administration reduces oxidative stress in erythrocytes of patients with polycystic ovary syndrome. Eur J Endocrinol 166(4):703–710
Ghorbani M, Vatannejad A, Khodadadi I, Amiri I, Tavilani H (2016) Protective effects of glutathione supplementation against oxidative stress during cryopreservation of human spermatozoa. Cryoletters 37(1):34–40
Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, Shahverdi A (2018) Sperm cryopreservation: a review on current molecular cryobiology and advanced approaches. Reprod Biomed Online 37:327–339
Huang JYJ, Rosenwaks Z (2014) Assisted reproductive techniques. In: Human fertility. Springer, pp 171–231
Jiang WD, Kuang SY, Liu Y, Jiang J, Hu K, Li SH, Zhou XQ (2013) Effects of myo-inositol on proliferation, differentiation, oxidative status and antioxidant capacity of carp enterocytes in primary culture. Aquac Nutr 19(1):45–53
Kalthur G, Raj S, Thiyagarajan A, Kumar S, Kumar P, Adiga SK (2011) Vitamin E supplementation in semen-freezing medium improves the motility and protects sperm from freeze-thaw–induced DNA damage. Fertil Steril 95(3):1149–1151
Karimfar M, Niazvand F, Haghani K, Ghafourian S, Shirazi R, Bakhtiyari S (2015) The protective effects of melatonin against cryopreservation-induced oxidative stress in human sperm. Int J Immunopathol Pharmacol 28(1):69–76
Ko EY, Sabanegh ES Jr, Agarwal A (2014) Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril 102(6):1518–1527
Kopeika J, Thornhill A, Khalaf Y (2014) The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update 21(2):209–227
Korosi T, Barta C, Rokob K, Torok T (2017) Physiological Intra-Cytoplasmic Sperm Injection (PICSI) outcomes after oral pretreatment and semen incubation with myo-inositol in oligoasthenoteratozoospermic men: results from a prospective, randomized controlled trial. Eur Rev Med Pharmacol Sci 21(2 Suppl):66–72
Kumar R, Saxena V, Shamsi MB, Venkatesh S, Dada R (2011) Herbo-mineral supplementation in men with idiopathic oligoasthenoteratospermia: a double blind randomized placebo-controlled trial. Indian J Urol IJU J Urol Soc India 27(3):357
Machen GL, Harris SE, Bird ET, Brown ML, Ingalsbe DA, East MM, Kuehl TJ (2018) Utilization of cryopreserved sperm cells based on the indication for storage. Invest Clin Urol 59(3):177–181
Mahfouz RZ, Sharma RK, Said TM, Erenpreiss J, Agarwal A (2009) Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa. Fertil Steril 91(4):1110–1118
Meamar M, Zribi N, Cambi M, Tamburrino L, Marchiani S, Filimberti E, Forti G (2012) Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica. Fertil Steril 98(2):326–333
Mocé E, Fajardo AJ, Graham JK (2016) Human sperm cryopreservation. EMJ 1(1):86–91
Mohagheghi AM-J, Amanpour S (2015) Systematic review of available guidelines on fertility preservation of young patients with breast cancer. Asian Pac J Cancer Prev 16(3):1057–1062
Mohammadi F, Varanloo N, Nasrabadi MH, Vatannejad A, Amjadi F, Masroor MJ et al (2019) Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis. Cell Tissue Bank 20:1–10
Montanino Oliva M, Minutolo E, Lippa A, Iaconianni P, Vaiarelli A (2016) Effect of myoinositol and antioxidants on sperm quality in men with metabolic syndrome. Int J Endocrinol 2016:5
O’connell M, McClure N, Lewis S (2002) The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod 17(3):704–709
Palmieri M, Papale P, Della Ragione A, Quaranta G, Russo G, Russo S (2016) In vitro antioxidant treatment of semen samples in assisted reproductive technology: effects of myo-inositol on nemaspermic parameters. Int J Endocrinol 2016:5
Prakash S, Prithiviraj E, Suresh S, Lakshmi NV, Ganesh MK, Anuradha M, Dinesh P (2014) Morphological diversity of sperm: a mini review. Iran J Reprod Med 12(4):239
Ranganathan P, Mahran AM, Hallak J, Agarwal A (2002) Sperm cryopreservation for men with nonmalignant, systemic diseases: a descriptive study. J Androl 23(1):71–75
Rezaeian Z, Yazdekhasti H, Nasri S, Rajabi Z, Fallahi P, Amidi F (2016) Effect of selenium on human sperm parameters after freezing and thawing procedures. Asian Pac J Reprod 5(6):462–466
Said TM, Gaglani A, Agarwal A (2010) Implication of apoptosis in sperm cryoinjury. Reprod Biomed Online 21(4):456–462
Saleh R, Assaf H, El Maged A, Wafaa M, Elsuity M, Fawzy M (2018) Increased cryo-survival rate in ejaculated human sperm from infertile men following pre-freeze in vitro myo-inositol supplementation. Clin Exp Reprod Med 45(4):177–182
Shabani Nashtaei M, Amidi F, Sedighi Gilani MA, Aleyasin A, Bakhshalizadeh S, Naji M, Nekoonam S (2017) Protective features of resveratrol on human spermatozoa cryopreservation may be mediated through 5′AMP-activated protein kinase activation. Andrology 5(2):313–326
Turan P, Şentürk GE, Ercan F (2017) Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men. Marmara Med J 30(2):63–72
Vatannejad A, Tavilani H, Sadeghi MR, Amanpour S, Shapourizadeh S, Doosti M (2017) Evaluation of ROS-TAC score and DNA damage in fertile normozoospermic and infertile asthenozoospermic males. Urol J 14(1):2973–2978
Vatannejad A, Tavilani H, Sadeghi MR, Karimi M, Lakpour N, Amanpour S et al (2019) Evaluation of the NOX5 protein expression and oxidative stress in sperm from asthenozoospermic men compared to normozoospermic men. J Endocrinol Invest 42:1–9
Walczak-Jedrzejowska R, Wolski JK, Slowikowska-Hilczer J (2013) The role of oxidative stress and antioxidants in male fertility. Cent Eur J Urol 66(1):60–67. https://doi.org/10.5173/ceju.2013.01.art19
WHO (2010) WHO laboratory manual for the examination and processing of human semen
Zandieh Z, Vatannejad A, Doosti M, Zabihzadeh S, Haddadi M, Bajelan L et al (2018) Comparing reactive oxygen species and DNA fragmentation in semen samples of unexplained infertile and healthy fertile men. Ir J Med Sci 187(3):657–662
Acknowledgments
The authors would like to thank the Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran for their financial support (No. 32641).
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This study was funded by Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran (grant number 32641).
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Abdolsamadi, M., Mohammadi, F., Nashtaei, M.S. et al. Does myoinositol supplement improve sperm parameters and DNA integrity in patients with oligoasthenoteratozoospermia after the freezing–thawing process?. Cell Tissue Bank 21, 99–106 (2020). https://doi.org/10.1007/s10561-019-09801-7
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DOI: https://doi.org/10.1007/s10561-019-09801-7