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Oxidative stress and hematological profiles of advanced breast cancer patients subjected to paclitaxel or doxorubicin chemotherapy

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Abstract

Several adverse effects of chemotherapy treatments have been described, and most of these effects are associated with direct interactions between blood cells and indirect effects generated during the oxidative metabolism of antineoplastic drugs. In this study we evaluated the oxidative systemic status and hematological profiles of breast cancer patients with advanced ductal infiltrative carcinoma treated with doxorubicin (DOX) or paclitaxel (PTX) within 1 h after chemotherapy. Blood analyses included evaluation of hemogram, pro-oxidative markers, and antioxidant status. The results showed that advanced breast cancer diseased (AD) patients without previous chemotherapy presented anemia and high oxidative stress status characterized by elevated levels of lipid peroxidation and nitric oxide, and reduced catalase activity when compared with controls. DOX-treated patients exhibited increased anemia and reduced antioxidant status, which was revealed by decreases in reduced glutathione levels and the total antioxidant capacity of plasma; however, these changes did not lead to further increases in lipid peroxidation or carbonyl proteins when compared with the AD group. PTX-treated patients also showed increased anemia, lactate dehydrogenase leakage, and enhanced lipid peroxidation. These data reveal for the first time that patients subjected to chemotherapy with DOX or PTX present immediate systemic oxidative stress and red blood cell oxidative injury with anemia development. These findings provide a new perspective on the systemic redox state of AD and patients subjected to chemotherapy regarding oxidative stress enhancement and its possible involvement in the aggravation of chronic anemia.

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Abbreviations

AD:

Advanced breast cancer patients

DOX:

Doxorubicin

PTX:

Paclitaxel

LDH:

Lactate dehydrogenase

RBC:

Red blood cells

TNM:

Tumor node metastasis classification

MCV:

Mean cellular volume

SOD:

Superoxide dismutase

GSH:

Reduced glutathione

TCA:

Trichloroacetic acid

TRAP:

Total antioxidant capacity

ABAP:

2,2′Azobis

RLU:

Relative light unities

NO:

Nitric oxide

TBARS:

Thiobarbituric reactive substances

MDA:

Malondialdehyde

DNPH:

Dinitrophenylhydrazine

AUC:

Area under the curve

LDL:

Low density lipoprotein

CL:

Chemiluminescence

V0:

Initial velocity of CL reaction

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Acknowledgments

The authors thank Jesus Vargas for his exceptional technical assistance, and the Fundação Araucária, CNPq, and CAPES for providing financial support.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Laboratory of Pathophysiology and Free Radicals, Department of General Pathology—Center of Biological Science, State University of Londrina, Londrina, PR, 86051-990, Brazil

    C. Panis, A. C. S. A. Herrera, V. J. Victorino, F. C. Campos, L. F. Freitas, T. De Rossi, A. L. Cecchini & R. Cecchini

  2. University Hospital, Department of Pharmacy, State University of Londrina, Londrina, PR, Brazil

    A. N. Colado Simão

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  1. C. Panis
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  2. A. C. S. A. Herrera
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  8. A. L. Cecchini
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Correspondence to R. Cecchini.

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Panis, C., Herrera, A.C.S.A., Victorino, V.J. et al. Oxidative stress and hematological profiles of advanced breast cancer patients subjected to paclitaxel or doxorubicin chemotherapy. Breast Cancer Res Treat 133, 89–97 (2012). https://doi.org/10.1007/s10549-011-1693-x

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  • DOI: https://doi.org/10.1007/s10549-011-1693-x

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