biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 62:471-478, 2018 | DOI: 10.1007/s10535-018-0786-3

Comparative analysis of DNA methylation changes in two contrasting wheat genotypes under water deficit

A. Kaur1,2, A. Grewal2, P. Sharma1,*
1 Division of Crop Improvement, Indian Institute of Wheat and Barley Research, ICAR, Karnal, India
2 Division of Biotechnology, University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra, India

DNA methylation is one of the epigenetic mechanisms regulating gene expression in plants in response to environmental conditions. In this study, analysis of methylation patterns was carried out in order to assess the effect of water stress in two contrasting wheat genotypes using methylation-sensitive amplified polymorphism (MSAP). The results revealed that demethylation was higher in drought-tolerant genotype (C306) as compared to drought-sensitive genotype (HUW468) after experiencing drought stress. Comparisons of different MSAP patterns showed a high percentage of polymorphic bands between tolerant and susceptible wheat genotypes (from 74.79 % at anthesis to 88.89 % at tillering). Furthermore, differential DNA methylation in roots and leaves also revealed tissue-specific methylation of genomic DNA. Interestingly, 54 developmental stage-specific bands and 23 bands that were found contrasting between these two wheat genotypes were detected. Furthermore, a few sites with stable DNA methylation differences were identified between drought-tolerant and drought-sensitive cultivars, thus providing genotype-specific epigenetic markers. These results not only provide data on differences in DNA methylation changes but also contribute to dissection of molecular mechanisms of drought response and tolerance in wheat.

Keywords: drought tolerance; methylation-sensitive amplified polymorphism; Triticum aestivum
Subjects: DNA methylation; water stress; drought tolerance; plant development; methylation-sensitive amplified polymorphism; relative water content; wheat

Received: May 19, 2017; Revised: October 28, 2017; Accepted: November 3, 2017; Published: September 1, 2018  Show citation

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Kaur, A., Grewal, A., & Sharma, P. (2018). Comparative analysis of DNA methylation changes in two contrasting wheat genotypes under water deficit. Biologia plantarum, 62(3), 471-478. doi: 10.1007/s10535-018-0786-3
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