biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:115-126, 2017 | DOI: 10.1007/s10535-016-0679-2

RETRACTED: Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses

M. Migocka1,*, A. Papierniak1, A. Rajsz1
1 Department of Plant Molecular Physiology, Institute of Experimental Biology, Wrocław University, Wrocław, Poland

The cucumber genes CsPDR8/CsABCG36 and CsPDR12/CsABCG40 encode two similar pleiotropic drug resistance proteins (ABCGPDRs) belonging to the large ABC family of multispecific ATP-dependent transporters. We have already shown that the amount of root CsPDR8/CsABCG36 and CsPDR12/CsABCG40 transcripts is markedly elevated by phytohormones related to the plant response to environmental constraints, suggesting the involvement of both genes in hormone-mediated reactions to stresses. To further characterize the function and regulation of CsPDR8/CsABCG36 and CsPDR12/CsABCG40, we determined the subcellular localization of the predicted CsPDR8/CsABCG36 and CsPDR12/CsABCG40 proteins in cucumber and performed a transcriptional analysis of genes encoding these proteins under different abiotic stresses (heavy metals, salinity, osmotic stress, and oxidative stress) and redox perturbations. In addition, the activities of antioxidative enzymes as well as the content of hydrogen peroxide and superoxide were measured in cucumber roots to monitor the redox perturbations under all experimental conditions. Western blot analysis of membrane fractions prepared from cucumber roots with specific antibodies raised against the peptides corresponding to sequences unique to CsPDR8/CsABCG36 and CsPDR12/CsABCG40 revealed that both proteins localize to the plasma membrane. The transcript abundance and the plasma membrane protein content closely correlated with the stress severity and the hydrogen peroxide content but not with the superoxide anion content. Based on the results obtained so far, we may conclude that CsPDR8/CsABCG36 and CsPDR12/CsABCG40 are up-regulated under multiple stress conditions and redox perturbations and that the H2O2 and stress-related phytohormones can act as signaling molecules affecting the expression of both cucumber genes.

Keywords: gene expression; heavy metals; osmotic stress; oxidative stress; reactive oxygen species; salt stress
Subjects: plasma membrane proteins; heavy metals; osmotic stress; oxidative stress; salt stress; gene expression; polyethylene glycol; mannitol; cadmium; lead; copper; zinc; nickel; manganese; ascorbate; glutathione; catalase; ascorbate peroxidase; glutathione reductase; hydrogen peroxide; cucumber

Received: October 26, 2015; Revised: May 18, 2016; Accepted: June 2, 2016; Published: January 1, 2017  Show citation

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Migocka, M., Papierniak, A., & Rajsz, A. (2017). RETRACTED: Cucumber PDR8/ABCG36 and PDR12/ABCG40 plasma membrane proteins and their up-regulation under abiotic stresses. Biologia plantarum, 61(1), 115-126. doi: 10.1007/s10535-016-0679-2

Link to retraction of this article: BIOLOGIA PLANTARUM (2021) 65: 359-359

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    References

    1. Bowler, C., Montagu, M. V., Inze, D.: Superoxide dismutase and stress tolerance. - Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83-116, 1992. Go to original source...
    2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal Biochem. 72: 248-254, 1976. Go to original source...
    3. Burke, J.J., Gamble, P.E., Hatfield, J.L., Quisenberry, J.E.: Plant morphological and biochemical responses to field water deficits. - Plant Physiol. 79: 415-419, 1985. Go to original source...
    4. Campbell, E.J., Schenk, P.M., Kazan, K., Penninckx, I.A., Anderson, J.P., Maclean. D.J., Cammue B.P.A., Ebert P.R., Manners J.M.: Pathogen-responsive expression of a putative ATP-binding cassette transporter gene conferring resistance to the diterpenoid sclareol is regulated by multiple defense signaling pathways in Arabidopsis. - Plant Physiol. 133: 1272-1284, 2003. Go to original source...
    5. Carlberg, I., Mannervik, B.: Purification and characterization of the flavoenzyme glutathione reductase from rat liver. - J. biol. Chem. 250: 5475-5480, 1975. Go to original source...
    6. Chen, G., Asada, K.: Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. - Plant Cell Physiol. 30: 987-998, 1989.
    7. Crouzet, J., Trombik, T., Fraysse, Å.S., Boutry, M.: Organization and function of the plant pleiotropic drug resistance ABC transporter family. - FEBS Lett. 580: 1123-1130, 2006. Go to original source...
    8. Czechowski, T., Stitt, M., Altmann, T., Udvardi, M.K., Scheible, W.R.: Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. - Plant Physiol 139: 5-17, 2005. Go to original source...
    9. Drazkiewicz, M., Skórzynska-Polit, E., Krupa, Z.: Copperinduced oxidative stress and antioxidant defence in Arabidopsis thaliana. - Biometals 17: 379-387, 2004. Go to original source...
    10. Eichhorn, H., Klinghammer, M., Becht, P., Tenhaken, R.: Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid. - J. exp. Bot. 57: 2193-2201, 2006. Go to original source...
    11. Foyer, C.H.: Oxygen metabolism and electron transport in photosynthesis. - In: Foyer, C.H. (ed.): Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Pp. 587-621. CSHL Press, Cold Spring Harbor 1997.
    12. Jabs, T., Dietrich, R.A., Dangl, J.L.: Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. - Science 273: 1853-1856, 1996. Go to original source...
    13. Kabala, K., Klobus, G.: Characterization of the tonoplast proton pumps in Cucumis sativus L. root cells. - Acta Physiol Plant. 23: 55-63, 2001. Go to original source...
    14. Kang, J., Hwang, J.-U., Lee, M., Kim, Y.-Y., Assmann, S.M., Martinoia, E., Lee Y: PDR-type ABC transporter mediates cellular uptake of the phytohormone abscisic acid. - Proc. nat. Acad. Sci. USA 107: 2355-2360, 2010. Go to original source...
    15. Kim, D.Y., Bovet, L., Maeshima, M., Martinoia, E., Lee, Y.: The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. - Plant J. 50: 207-218, 2007. Go to original source...
    16. Kim, D.Y., Jin, J.Y., Alejandro, S., Martinoia, E., Lee, Y.: Overexpression of AtABCG36 improves drought and salt stress resistance in Arabidopsis. - Physiol Plant. 139: 170-180, 2010. Go to original source...
    17. Kobae, Y., Sekino, T., Yoshioka, H., Nakagawa, T., Martinoia, E., Maeshima, M.: Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. - Plant Cell Physiol. 47: 309-318, 2006. Go to original source...
    18. Kretzschmar, T., Kohlen, W., Sasse, J., Borghi, L., Schlegel, M., Bachelier, J.B., Reinhardt D., Bours R., Bouwmeester H.J., Martinoia E.: A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching. - Nature 483: 341-344, 2012. Go to original source...
    19. Lee, M., Lee, K., Lee, J., Noh, E.W., Lee, Y.: AtPDR12 contributes to lead resistance in Arabidopsis. - Plant Physiol. 138: 827-836, 2005. Go to original source...
    20. Levine, A., Tenhaken, R., Dixon, R., Lamb, C.: H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. - Cell 79: 583-593, 1994. Go to original source...
    21. Lopez-Delgado, H., Dat, J.F., Foyer, C.H., Scott, I.M.: Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2. - J. exp. Bot. 49: 713-720, 1998. Go to original source...
    22. Migocka, M., Klobus, G.: The properties of the Mn, Ni and Pb transport operating at plasma membranes of cucumber roots. - Physiol Plant. 129: 578-587, 2007. Go to original source...
    23. Migocka, M., Papierniak, A.: Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators. - Mol. Breed. 28: 343-357, 2011. Go to original source...
    24. Migocka, M., Papierniak, A., Kosatka, E., Klobus, G.: Comparative study of the active cadmium efflux systems operating at the plasma membrane and tonoplast of cucumber root cells. - J. exp. Bot. 62: 4903-4916, 2011. Go to original source...
    25. Migocka, M., Papierniak, A., Warzybok, A., Klobus, G.: CsPDR8 and CsPDR12, two of the 16 pleiotropic drug resistance genes in cucumber, are transcriptionally regulated by phytohormones and auxin herbicide in roots. - Plant Growth Regul. 67: 171-184, 2012. Go to original source...
    26. Moons, A.: Ospdr9, which encodes a PDR-type ABC transporter, is induced by heavy metals, hypoxic stress and redox perturbations in rice roots. - FEBS Lett. 553: 370-376, 2003. Go to original source...
    27. Moons, A.: Transcriptional profiling of the PDR gene family in rice roots in response to plant growth regulators, redox perturbations and weak organic acid stresses. - Planta 229: 53-71, 2008. Go to original source...
    28. Sasabe, M., Toyoda, K., Shiraishi, T., Inagaki, Y., Ichinose, Y.: cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene. - FEBS Lett. 518: 164-168, 2002. Go to original source...
    29. Scandalios, J.G.: Molecular responses to oxidative stress. - In: Hawkesford, M.J., Buchner, P.C. (ed.): Molecular Analysis of Plant Adaptation to the Environment. Pp. 181-208. Springer, Dordrecht 2001. Go to original source...
    30. Scandalios, J.G.: Molecular genetics of superoxide dismutases. - In: Scandalios, J.G. (ed.): Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Pp. 527-568. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1997.
    31. Scott-Craig, J.S., Kerby, K.B., Stein, B.D., Somerville, S.C.: Expression of an extracellular peroxidase that is induced in barley (Hordeum vulgare) by the powdery mildew pathogen (Erysiphe graminis f. sp. hordei). - Physiol. mol. Plant Pathol. 47: 407-418, 1995. Go to original source...
    32. Sharma, Y.K., Léon, J., Raskin, I., Davis, K.R.: Ozone-induced responses in Arabidopsis thaliana: the role of salicylic acid in the accumulation of defense-related transcripts and induced resistance. - Proc. nat. Acad. Sc.i USA 93: 5099-50104, 1996. Go to original source...
    33. Summers, L.A. (ed.): The Bipyridylium Herbicides. - Academic Press, London 1980.
    34. Thordal-Christensen, H., Brandt, J., Cho, B.H., Rasmussen, S.K., Gregersen, P.L., Smedegaard-Petersen V., Collinge, D.B.: cDNA cloning and characterization of two barley peroxidase transcripts induced differentially by the powdery mildew fungus Erysiphe graminis. - Physiol. mol. Plant Pathol. 40: 395-409, 1992. Go to original source...
    35. Trombik, T., Jasinski, M., Crouzet, J., Boutry, M.: Identification of a cluster IVpleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia. - Plant mol. Biol. 66: 165-175, 2008. Go to original source...
    36. Van Den Brûle, S., Smart, C.C.: The plant PDR family of ABC transporters. - Planta 216: 95-106, 2002. Go to original source...
    37. Warzybok, A., Migocka, M.: Reliable reference genes for normalization of gene expression in cucumber grown under different nitrogen nutrition. - PLoS ONE 8: e72887, 2013. Go to original source...
    38. Xi, J., Xu, P., Xiang, C.,B.: Loss of AtPDR11, a plasma membrane-localized ABC transporter, confers paraquat tolerance in Arabidopsis thaliana. - Plant J. 69: 782-791, 2012. Go to original source...

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