biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 60:628-634, 2016 | DOI: 10.1007/s10535-016-0638-y

Age-associated alterations in DNA methylation and expression of methyltransferase and demethylase genes in Arabidopsis thaliana

Z. V. Ogneva1,2, A. S. Dubrovina1, K. V. Kiselev1,2,*
1 Laboratory of Biotechnology, Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia
2 Department of Biochemistry and Microbiology, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia

Little is known about the contributions of DNA methylation/demethylation to plant aging and senescence. We used Arabidopsis thaliana to study how increasing age of an annual plant species influences DNA methylation. Based on methylation-sensitive DNA fragmentation assay, it could be concluded that aging A. thaliana was accompanied by DNA demethylation. Bisulfite sequencing reveals that cytosine methylation within the Actin2 3' untranslated region and internal transcribed spacer with 5.8S rRNA (ITS1-5.8SrRNA-ITS2) DNA regions decreased with A. thaliana growth and aging. We show that transcription of methyltransferase genes, chromomethyltransferase AtCMT3 and methyltransferse AtMETI, significantly decreased during development and aging of the A. thaliana plants, whereas expression of demethylase genes - repressor of silencing AtROS1, demeter AtDME, and demeter-like AtDML2 and AtDML3 - increased at least at some stages of plant development. The data obtained in the present study suggest that plant DNA regions may undergo demethylation during plant aging via reduction of DNA methylation processes and activation of active DNA demethylation.

Keywords: bisulfite sequencing; methylation-sensitive DNA fragmentation assay; plant ageing
Subjects: DNA methylation; methyltransferase; demethylase; DNA fragmentation; plant ageing; gene expression

Received: August 15, 2015; Revised: December 19, 2015; Accepted: December 29, 2015; Published: December 1, 2016  Show citation

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Ogneva, Z.V., Dubrovina, A.S., & Kiselev, K.V. (2016). Age-associated alterations in DNA methylation and expression of methyltransferase and demethylase genes in Arabidopsis thaliana. Biologia plantarum, 60(4), 628-634. doi: 10.1007/s10535-016-0638-y
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