biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 59:637-644, 2015 | DOI: 10.1007/s10535-015-0531-0

Analysis of the flanking sequences of the heterochromatic JNK region in Secale vavilovii chromosomes

A. Kalinka1,2, M. Achrem1,2,*
1 Department of Cell Biology, University of Szczecin, Szczecin, Poland
2 Molecular Biology and Biotechnology Center, Faculty of Biology, University of Szczecin, Szczecin, Poland

The JNK motif is repeated 4 000 times in an additional heterochromatin band. Flanking sequences of a JNK region, which form extra heterochromatin in 2R rye (Secale vavilovii Grossh.) chromosomes, were studied using a genome walking technique. The results clearly indicate that there were blocks of JNK sequences adjacent to the R173 family of repeated sequences. Moreover, it appears that the R173 are sequences flanking in both directions, i.e., upstream and downstream. Downstream, the R173 is adjacent to the JNKs in an anti-parallel orientation, whereas upstream it is adjacent in a parallel orientation. In order to confirm the presence of the R173 sequence, fluorescence in situ hybridization was carried out. Using both JNK and R173 molecular probes, overlapping hybridization signals in the 2RL pair of chromosomes were observed, indicating an identical location of the two sequence elements.

Keywords: FISH; genome walking; repeated sequences; rye
Subjects: flanking sequences; heterochromatin; FISH; genome walking; repeated sequences; rye

Received: October 10, 2014; Revised: April 8, 2015; Accepted: April 28, 2015; Published: December 1, 2015  Show citation

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Kalinka, A., & Achrem, M. (2015). Analysis of the flanking sequences of the heterochromatic JNK region in Secale vavilovii chromosomes. Biologia plantarum, 59(4), 637-644. doi: 10.1007/s10535-015-0531-0
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