biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:641-648, 2014 | DOI: 10.1007/s10535-014-0452-3

Molecular cloning and expression analyses of FaFT, FaTFL, and FaAP1 genes in cultivated strawberry: their correlation to flower bud formation

R. Nakajima1, S. Otagaki1, K. Yamada2, K. Shiratake1, S. Matsumoto1,*
1 Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan
2 College of Bioscience and Biotechnology, Chubu University, Kasugai, Japan

In this study, we cloned flowering-related genes FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) from domesticated octaploid strawberries (Fragaria × ananassa) and analyzed their expression patterns in cultivars Tochiotome and Akihime. The floral meristem generation was induced under the short day and low temperature (SDLT), but not under the long day and high temperature (LDHT). We found that FaFT1, which is an orthologue of the Arabidopsis floral activator FT, was highly expressed in leaves under LDHT but not expressed in leaves under SDLT. On the other hand, the expression of FaTFL2, which belongs to the TFL1 family of flowering repressing genes, decreased in crowns (stem tissue including meristem) under SDLT. These results suggest that FaTFL2, as opposed to FvTFL1 in wild diploid strawberry Fragaria vesca, is related to flowering of the cultivated strawberry. Moreover, the FaTFL2 expression might be regulated by temperature rather than by photoperiod. We demonstrated that a reduction of the FaTFL2 expression is a key signal for flowering in domesticated strawberries.

Keywords: flowering; Fragaria × ananassa; F. vesca; photoperiod; temperature
Subjects: flowering-related genes; amino acid sequences; phylogenetic tree; gene expression; circadian rhythm; flowering; strawberry

Received: October 17, 2013; Revised: February 7, 2014; Accepted: February 14, 2014; Published: December 1, 2014  Show citation

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Nakajima, R., Otagaki, S., Yamada, K., Shiratake, K., & Matsumoto, S. (2014). Molecular cloning and expression analyses of FaFT, FaTFL, and FaAP1 genes in cultivated strawberry: their correlation to flower bud formation. Biologia plantarum, 58(4), 641-648. doi: 10.1007/s10535-014-0452-3
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