biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:283-291, 2012 | DOI: 10.1007/s10535-012-0088-0

Genetic diversity in white clover and its progenitors as revealed by DNA fingerprinting

A. Badr1,*, H.H. El-Shazly2, L. Mekki3
1 Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
2 Faculty of Education, Ain Shams University, Roxy, Heliopolis, Cairo, Egypt
3 Botany Department, Faculty of Science, Suiz Canal University, Ismailia, Egypt

The genetic diversity and ancestral relationships of a number of Trifolium species was revealed by using the amplified fragment length polymorphism (AFLP) and the random amplified polymorphic DNA (RAPD) markers. Both markers produced few species-specific markers. Using distance and parsimony methods, in NTSYS-pc and PAUP software programs, we clearly differentiated the accessions of white clover from other closely related progenitors. The phylogenetic trees, produced by PAUP, also reinforced the close affinity of T. nigrescens and the allopolyploid white clover in support of former views that this diploid species could have been the donor of one of two genomes of the allotetraploid T. repens. In addition, the dendrograms, produced by NTSYS-pc, also indicated close affinity of T. nigrescens and T. occidentale to the accessions of T. repens. These data is congruent with karyological and phylogenetic affinities between the white clover and T. occidentale. The relationships between the examined accessions, in the T. repens gene pool, may be regarded to indicate the presence of shared alleles between T. repens, T. occidentale and T. uniflorum. Further, T. occidentale showed close phylogenetic relations to T. pallescens.

Keywords: AFLP; ancestry; progenitors; RAPD; Trifolium repens
Subjects: AFLP; ancestry; progenitors; RAPD; genetic diversity; DNA fingerprinting; phylogenetic tree

Received: May 1, 2010; Accepted: November 12, 2010; Published: June 1, 2012  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Badr, A., El-Shazly, H.H., & Mekki, L. (2012). Genetic diversity in white clover and its progenitors as revealed by DNA fingerprinting. Biologia plantarum, 56(2), 283-291. doi: 10.1007/s10535-012-0088-0
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abberton, M.T.: Interspecific hybridization in the genus Trifolium. - Plant Breed. 126: 337-342, 2007. Go to original source...
    2. Abberton, M.T., Marshal, A.H.: Progress in breeding perennial clovers for temperate agriculture. - Centenary Rev. J. agr. Sci. 143: 117-135., 2005. Go to original source...
    3. Ansari, H.A., Ellison, N.W., Griffiths, A.G., Williams, W.M.: A lineage-specific centromeric satellite sequence in the genus Trifolium. - Chromosome Res. 12: 357-367, 2004. Go to original source...
    4. Ansari, H.A., Ellison, N.W., Reader, S.M., Badeva, E.D., Friebe, B., Miller, T.E., Williams, W.M.: Molecular cytogenetic organization of 5S and 18S-26S rDNA loci in white clover (Trifolium repens L) and related species. - Ann. Bot. 83:199-206, 1999. Go to original source...
    5. Badr, A., Sayed-Ahmed, H., El-Shanshouri, A., Watson, L.E.: Ancestors of white clover (Trifolium repens L.), as revealed by isozyme polymorphisms. - Theor. appl. Genet. 106: 143-148, 2002. Go to original source...
    6. Badr, A., El-Shazly, H.H., Watson, L.E.: Origin and ancestry of Egyptian clover (Trifolium alexandrinum L.) as revealed by AFLP markers. - Genet. Res. Crop Evol. 55: 21-31, 2008. Go to original source...
    7. Badr, A., Müller, K., Schäfer-Pregl, R., El-Rabey, H., Effgen, S., Ibrahim, H.H., Pozzi, C., Rohde, W., Salamini. F.: On the origin and domestication history of barley (Hordeum vulgare L.). - Mol. Biol. Evol. 17: 499-510, 2000. Go to original source...
    8. Bortolini, F., Miguel, D., Schifino-Wittmann, M.: Molecular characterization of the USDA white clover (Trifolium repens L.) core collection by RAPD markers. - Genet. Res. Crop Evol. 53: 1081-1087, 2006. Go to original source...
    9. Brewbaker, J.L., Keim, W.F.: A fertile interspecific hybrid in Trifolium. - Amer. Natur. 87: 323-326, 1953. Go to original source...
    10. Chen, C.C., Gibson, P.B.: Chromosome pairing in two interspecific hybrids of Trifolium. - Can. J. Genet. Cytol. 12: 190-794, 1970. Go to original source...
    11. Chen, C.C., Gibson, P.B.: Barriers to hybridization of Trifolium repens with related species. - Can. J. Genet. Cytol. 14: 591-595, 1972. Go to original source...
    12. Darlington, C.D., La Cour, F.: Handling of Chromosomes. 6th Ed. - George Allen and Unwin, London.
    13. Ellison, N.W., Liston, A., Steiner, J.J., Williams, W.M., Taylor, N.L.: Molecular phylogenetics of the clover genus (Trifolium-Leguminosae). - Mol. Phylogen. Evol. 39: 688-705, 2006. Go to original source...
    14. Felsenstein, J.: Confidence limits on phylogenies: an approach using the bootstrap. - Evolution 39: 783-791, 1985. Go to original source...
    15. Gibson, P.G., Beinhart, G.: Hybridization of Trifolium occidentale with two other species of clover. - J. Hered. 60: 93-96, 1969. Go to original source...
    16. Gillett, J.M., Taylor, N.L.: The World of Clovers. - Iowa State University Press, Ames 2001.
    17. Hand, M.L., Ponting, R.C., Drayton, M.C., Lawless, K.A., Cogan, N.O.I., Brummer, C.E., Sawbridge, T.I., Spangenberg, G.C., Smith, K.F. Forster, J.W.: Identification of homologous, homoeologous and paralogous sequence variants in an outbreeding allopolyploid species based on comparison with progenitor taxa. - Mol. Genet. Genom. 280: 293-304, 2008. Go to original source...
    18. Hargreaves, S., Maxted, N., Hirano, R., Abberton, M., Skøt, L., Ford-Lloyd, B.V.: Islands as refugia of Trifolium repens genetic diversity. - Conserv. Genet. 11: 1317-1326, 2009. Go to original source...
    19. Heun, M., Schäfer-Pregl, R., Klawan, D., Castagna, R., Accerbi, M., Borghi, B., Salamini, F.: Site of einkorn wheat domestication identified by DNA finger-printing. - Science 278: 1312-1314, 1997. Go to original source...
    20. Hussain, S.W., Williams, W.M.: Evidence of functional unreduced gametes in Trifolium repens L. - Euphytica 97: 21-24, 1997. Go to original source...
    21. Hussain, S.W., Williams, W.M., Mercer, C.F., White. D.W.R.: Transfer of clover cyst nematode resistance from Trifolium nigrescens Viv. to T. repens by interspecific hybridisation. - Theor. appl. Genet. 95: 1274-1281, 1997. Go to original source...
    22. Jahufer, M.Z.Z., Cooper, M., Ayres, J.F., Bray, R.A.: Identification of research to improve the efficiency of breeding strategies for white clover in Australia: a review. - Aust. J. agr. Res. 53: 239-257, 2002. Go to original source...
    23. Kakes, P., Chardonnens, A.N.: Cyanotypic frequencies in adjacent and mixed populations of Trifolium occidentale Coombe and Trifolium repens L. are regulated by different mechanisms. - Biochem. Syst. Ecol. 28: 633-649, 2000. Go to original source...
    24. Kakes, P., Hakvoort, H.W.J.: On the origin of the cyanogenic polymorphism in Trifolium repens L. - J. Evol. Biol. 7: 201-215, 1994. Go to original source...
    25. Kazimierski, T., Kazimierska, E.M.: Investigation of hybrids in the genus Trifolium L. V. Fertility and cytogenetics of the hybrid Trifolium nigrescens and T. isthmocarpum. Brot. - Acta. Soc. Bot. Pol. 42: 567-589, 1973. Go to original source...
    26. Marshall, A.H., Abberton, M.T., Michaelson-Yeates, T.P.T., Bowen, C.: The application of molecular markers to monitor introgression of seed yield traits from Trifolium nigrescens into T. repens (white clover). - In: D. Loch (ed.): Proc. 5th International Herbage Seed Conference. Pp 79-83. IHSG, Gatton 2003.
    27. Marshall, A.H., Holdbrook-Smith, K., Michaelson-Yeates, T.P.T., Abberton, M.T., Rhodes, I.: Growth and reproductive characteristics in backcross hybrids derived from Trifolium repens L. × T. nigrescens Viv. interspecific crosses. - Euphytica 104: 61-66, 1998. Go to original source...
    28. Marshall, A.H., Michaelson-Yeates, T.P.T., Abberton, M.T.: Introgression of reproductive traits from Trifolium nigrescens increases the seed yield of white clover (T. repens). - Plant Breed. 127: 597-601, 2008. Go to original source...
    29. Marshall, A.H., Michaelson-Yeates, T.P.T., Aluka, P., Meredith, M.: Reproductive characters of interspecific hybrids between Trifolium repens L. and T. nigrescens Viv. - Heredity 74: 136-145, 1995. Go to original source...
    30. Marshall, A.H., Williams, T.A., Olyott, P., Abberton, M.T., Michaelson-Yeates, T.P.T.: Forage yield and persistency of Trifolium repens × Trifolium nigrescens hybrids under rotational sheep grazing. - Grass Forage Sci. 60: 68-73, 2005. Go to original source...
    31. Michaelson-Yeates, T.P.T., Marshall, A.H., Abberton, M.T.: Self-incompatibility and heterosis in white clover (Trifolium repens L.). - Euphytica 94: 341-348, 1997. Go to original source...
    32. Mo, X.Y., Long, T., Liu, Z., Lin, H., Liu, X.Z., Yang, Y.M., Zhang, H.Y. - AFLP analysis of somaclonal variations in Eucalyptus globules. Biol. Plant. 53: 741-744, 2009. Go to original source...
    33. Olsen, K.M., Sutherland, B.L., Small, L.L.: Molecular evolution of the Li/li chemical defense polymorphism in white clover (Trifolium repens L.). - Mol. Ecol. 16: 4180-4193, 2007. Go to original source...
    34. Piluzza, G., Pecetti, L., Bullitta, S., Piano, E.: Discrimination among subterranean clover (Trifolium subterraneum L. complex) accessions using RAPD markers. - Genet. Res. Crop. Evol. 52: 193-199, 2005. Go to original source...
    35. Rohlf, F.J.: NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.1. - Applied Biostatistics, New York 2002.
    36. Saghai-Maroof, N.A., Soliman, K.M., Jorgensen, R.A., Allard, R.: Ribosomal RNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. nat. Acad. Sci. USA 81: 8014-8018, 1984. Go to original source...
    37. Saitou, N., Nei, M.: The neighbor-joining method: a new method for reconstructing phylogenetic trees. - Mol. Biol. Evol. 4: 406-425, 1987.
    38. Sciacca, F., Fichera, C., Di Silvestro, S., Conte, E., Palumbo, M.: Genetic diversity of durum wheat as determined by AFLP in fluorescence. - Biol. Plant. 54: 435-442, 2010. Go to original source...
    39. Sokal, R.R., Michener, C.D.: A statistical method for evaluating systematic relationships. - Univ. Kansas Sci. Bull. 28: 1409-1438, 1958.
    40. Sprent, J.I.: Nodulation in Legumes. - Royal Botanic Gardens, Kew 2001.
    41. Swofford, D.L.: PAUP* Phylogenetic Analysis Using Parsimony* and Other Methods, Version 4. - Sinauer Associates, Sunderland 2002.
    42. Vos, P., Hogers, R., Bleckers, M., Beijans, M., Van de Lee, T., Hornes, M., Frijiters, A., Pot. J., Oeleman, J., Zabeau, M.: AFLP: New technique for DNA fingerprinting. - Nucl. Acids Res. 23: 4407-4414, 1995. Go to original source...
    43. Williams, J.G.K., Kubelk, R., Livark, K.J., Rafalski, J.A. Tingey, S.V.: DNA polymorphism amplified by arbitrary primers are useful as genetic markers. - Nucl. Acids Res. 18: 6231-6235, 1990. Go to original source...
    44. Williams, W.M.: Genetics and breeding. - In: Baker, M.J., Williams, W.M. (ed.): White Clover. Pp. 343-419. CAB International, Wallingford - Oxon 1987.
    45. Williams, W.M., Ansari, H.A., Ellison, N.W., Hussain, S.W.: Evidence of three subspecies in Trifolium nigrescens Viv. - Ann. Bot. 87: 683-691, 2001. Go to original source...
    46. Williams, W.M., Ansari, H.A., Hussain, S.W., Ellison, N.W., Williamson, M.L., Verry, I.M.: Hybridization and introgression between two diploid wild relatives of white clover, Trifolium nigrescens Viv. and T. occidentale Coombe. - Crop Sci. 48: 139-148, 2008. Go to original source...
    47. Zabeau, M., Vos, P.: Selective restriction fragment amplification: a general method for DNA fingerprinting. - European Patent Application No. 92402629-7, 1993.
    48. Zohary, M., Heller, D.: The Genus Trifolium. - The Israel Academy of Science and Humanities, Jerusalem 1984.

    Return to the content