biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:545-550, 2012 | DOI: 10.1007/s10535-011-0216-2

Chalcone isomerase in grape vine: gene expression and localization in the developing fruit

W. Wang1,3, H. -L. Wang1,2, S. -B. Wan1,4, J. -H. Zhang1, P. Zhang1, J. -C. Zhan1, W. -D. Huang1,2,*
1 Center for Research and Development of Viticulture and Enology, China Agricultural University, Beijing, P.R. China
2 Beijing Key Laboratory of Viticulture and Enology, Beijing, China
3 The Institute of Forestry, The Chinese Academy of Forestry, Beijing, P.R. China
4 School of Life Science, Shanghai University, Shanghai, P.R. China

Chalcone isomerase (CHI, EC 5.5.1.6) is an entrance enzyme in the flavonoid biosynthesis, which catalyzes the conversion of chalcones to flavanones. In this study, the full-length CHI cDNA from grape vine (Vitis vinifera L.) was cloned, the recombinant protein was purified and the polyclonal antibody was prepared. Using these tools, the expression and tissue localization of CHI in developing grape berry was analyzed by RT-PCR, gel blot hybridization and immunohistochemical techniques. The expression of CHI was dependent on developmental stage, and CHI protein was mainly distributed in vascular bundles throughout all the stages of berry development, which suggested that flavonoids in the berry might have been partially synthesized in situ.

Keywords: antibody; Escherichia coli; flavonoids; immunohistochemical technique; RT-PCR; Vitis vinifera
Subjects: flavonoids; immunohistochemical technique; PCR; chalcone isomerase; gene expression; fruit development; temperature - high

Received: January 27, 2010; Accepted: June 7, 2011; Published: September 1, 2012  Show citation

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Wang, W., Wang, H.-L., Wan, S.-B., Zhang, J.-H., Zhang, P., Zhan, J.-C., & Huang, W.-D. (2012). Chalcone isomerase in grape vine: gene expression and localization in the developing fruit. Biologia plantarum, 56(3), 545-550. doi: 10.1007/s10535-011-0216-2
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