biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:415-422, 2010 | DOI: 10.1007/s10535-010-0076-1

Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances

M. L. Osório1,*, J. Osório2, A. Romano1
1 IBB/CGB, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal
2 ICAAM, Faculty of Sciences and Technology, University of Algarve, Faro, Portugal

The aim of this study was to investigate acclimation of micropropagated plants of Rhododendron ponticum subsp. baeticum to different irradiances and recovery after exposure to high irradiance. Plants grown under high (HL) or intermediate (IL) irradiances displayed higher values of maximum electron transport rate (ETRmax) and light saturation coefficient (Ek) than plants grown under low irradiance (LL). The capacity of tolerance to photoinhibition (as assessed by the response of photochemical quenching, qp) varied as follows: HL > IL > LL. Thermal energy dissipation (qN) was also affected by growth irradiance, with higher saturating values being observed in HL plants. Light-response curves suggested a gradual replacement of qp by qN with increasing irradiance. Following exposure to irradiance higher than 1500 μmol m-2 s-1, a prolonged reduction of the maximal photochemical efficiency of PS 2 (Fv/Fm) was observed in LL plants, indicating the occurrence of chronic photoinhibition. In contrary, the decrease in Fv/Fm was quickly reverted in HL plants, pointing to a reversible photoinhibition.

Keywords: electron transport; photochemical quenching; photoinhibition; thermal energy dissipation
Subjects: chlorophyll fluorescence; electron transfer rate; ex vitro transfer; photoinhibition; photon flux density; photosynthetic rate; photosystems (PS 1, PS 2); Rhododendron pontium; thermal energy dissipation

Received: October 21, 2008; Accepted: April 11, 2009; Published: September 1, 2010  Show citation

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Osório, M.L., Osório, J., & Romano, A. (2010). Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances. Biologia plantarum, 54(3), 415-422. doi: 10.1007/s10535-010-0076-1
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