biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:386-390, 2010 | DOI: 10.1007/s10535-010-0070-7

Proline accumulation and the expression of Δ1-pyrroline-5-carboxylate synthetase in two safflower cultivars

M. Thippeswamy1,*, P. Chandraobulreddy1, B. Sinilal1, M. Shiva Kumar1, Chinta Sudhakar1
1 Department of Botany, Sri Krishnadeveraya University, Anantapur, India

Proline (Pro) accumulation under water stress was measured in safflower (Carthamus tinctorius L.) drought tolerant cv. A1 and sensitive cv. Nira. Activities of pyrroline-5-carboxylate reductase (P5C reductase) and pyrroline-5-carboxylate synthetase (P5C synthetase), two enzymes involved in the Pro biosynthetic pathway were also estimated. Water stress resulted in a reduction in the leaf dry mass and chlorophyll content along with a gradual accumulation of Pro. RT-PCR results show higher expression of Δ1-pyrroline-5-carboxylate synthetase (p5cs) gene in correlation with up-regulated Pro accumulation in cv. A1. P5C reductase was found to be the Pro synthesis rate limiting whereas P5C synthetase did not show any specific response to the drought stress in both cultivars.

Keywords: Carthamus tinctorius; safflower; water stress
Subjects: Carthamus tinctorius; chlorophyll a,b; proline; proline biosynthesis; safflower; stress resistance; water stress

Received: August 13, 2008; Accepted: January 5, 2009; Published: June 1, 2010  Show citation

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Thippeswamy, M., Chandraobulreddy, P., Sinilal, B., Shiva Kumar, M., & Sudhakar, C. (2010). Proline accumulation and the expression of Δ1-pyrroline-5-carboxylate synthetase in two safflower cultivars. Biologia plantarum, 54(2), 386-390. doi: 10.1007/s10535-010-0070-7
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