biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:386-390, 2007 | DOI: 10.1007/s10535-007-0082-0

Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings

Y. Lei1, C. Yin1, J. Ren1, C. Li1,*
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, P.R. China

Effect of osmotic stress and sodium nitroprusside (SNP, NO donor) pretreatment on growth and proline metabolism of wheat seedlings was investigated. Polyethylene glycol 6000 treatment for 2, 4 and 6 d could be termed as mild, moderate and severe stress, respectively, according to decrease in the relative water content. Severe osmotic stress significantly decreased the growth and photochemical efficiency, and increased proline content due to activation of its synthesis. 0.2 mM SNP pretreatment enhanced growth of wheat seedlings, increased variable to maximum fluorescence ratio (Fv/Fm) and fluorescence yield, while decreased proline content. However, 2 mM SNP retarded the seedlings growth and chlorophyll a fluorescence, and increased proline accumulation. Our results showed that NO might be involved in the regulation of osmotic stress in a concentration-dependent manner.

Keywords: chlorophyll a fluorescence; exogenous nitric oxide; Triticum aestivum L
Subjects: abscisic acid (ABA); chlorophyll fluorescence; osmotic potential, stress; proline; relative water content (RWC); sodium nitroprusside; Triticum aestivum; wheat

Received: September 12, 2005; Accepted: April 21, 2006; Published: June 1, 2007  Show citation

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Lei, Y., Yin, C., Ren, J., & Li, C. (2007). Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings. Biologia plantarum, 51(2), 386-390. doi: 10.1007/s10535-007-0082-0
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