biologia plantarum

Biologia plantarum 50:118-122, 2006 | DOI: 10.1007/s10535-005-0084-8

In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli

V. Agrawal^1,*, P. R. Sardar¹

¹ Department of Botany, University of Delhi, Delhi, India

High efficiency shoot regeneration was achieved through leaflet and cotyledon derived calli in Cassia angustifolia - an important medicinal plant. Dark brown compact callus was induced at the cut ends of the explants on Murashige and Skoog's (MS) medium augmented with 1 µM N⁶-benzyladenine (BA) + 1 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Such callus pieces on transfer to cytokinins (BA or kinetin) supplemented medium differentiated shoots within 10 - 15 d. Of the two cytokinins, 5 µM BA was optimum for eliciting morphogenic response in 83.33 and 70.83 % cultures with an average of 4.16 ± 0.47 and 3.70 ± 0.56 shoots in cotyledon and leaflet derived calli, respectively. The addition of 0.5 µM α-naphthaleneacetic acid (NAA) to MS + 5 µM BA further elevated the maximum average number of shoots to 12.08 ± 1.04 and 5.37 ± 0.52 for cotyledon and leaflet calli, respectively. The excised shoots were transferred to a rooting medium containing either IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) or NAA. Nearly 95 % shoots developed an average of 5.4 ± 0.41 roots on half strength MS medium supplemented with 10 µM IBA.

Keywords: benzyladenine; indole-3-butyric acid; multiple shoots; naphthaleneacetic acid; rooting; senna

Subjects: auxins; Cassia angustifolia; cotyledon; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, micropropagation, propagation; medicinal plants; nutrient medium, Murashige and Skoog; senna

Received: November 25, 2003; Accepted: September 14, 2005; Published: March 1, 2006  Show citation