Abstract
Six cadmium (Cd)-resistant microbial strains were isolated and their ability to immobilise Cd2+ in soil investigated. Cd-1, Cd-2, Cd-5, and Cd-6 were identified as Stenotrophomonas sp., Cd-3 as Achromobacter sp., and Cd-7 as Staphylococcus sp. The six strains showed a wide adaptation range for salinity and a strong tolerance to Cd2+. The effects of the initial Cd2+ concentration (1–100 mg/L), duration (18–72 h), temperature (10–40 °C), and pH (5.0–9.0) on the efficiency of Cd2+ removal were analysed. The results revealed that the Cd2+ removal rate was higher at an initial Cd2+ concentration of 5–100 mg/L than at 1 mg/L. The maximum Cd2+ removal effect was at a culture duration of 36 h, temperature of 10–35 °C, and pH of 5.0–7.0. X-ray diffraction (XRD) analysis revealed that the Cd2+ was immobilised by Stenotrophomonas sp. Cd-2 and Staphylococcus sp. Cd-7 through bio-precipitation. X-ray photoelectron spectroscopy (XPS) revealed that the Cd2+ was adsorbed by Stenotrophomonas sp. Cd-2, Achromobacter sp. Cd-3, and Staphylococcus sp. Cd-7. Fourier transform infrared spectroscopy (FTIR) analysis revealed that the isolates reacted with the Cd2+ mainly through the O–H, protein N–H, C–N, lipid C–H, fatty acid COO, polysaccharide C–O, P–O, and other functional groups, as well as with lipid molecules on the cell wall surfaces. Scanning electron microscopy (SEM) analysis revealed that there was little difference in the cells after Cd2+ treatment. The results of the soil remediation experiments indicated that the toxicity of Cd in soil could be effectively reduced using certain strains of microbe.
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Funding
This work was financially supported by the Ningxia Natural Science Foundation (Excellent Youth Project) (No. 2021AAC05012), the National Natural Science Foundation of China (No. 21966001), and the Collaborative Heavy Metal Detection Project in Soil of Producing Area and Agricultural Products (2021–87).
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RF: conceptualization, methodology, resources, writing original–draft, writing–review & editing, supervision, project administration. WX: investigation, formal analysis, data curation. HM: validation, data curation. MZ: validation, visualization. KM: resources, writing–review & editing. XY: funding acquisition, writing–review & editing.
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Fan, R., Xie, W., Ma, H. et al. Isolation of cadmium-resistant microbial strains and their immobilisation of cadmium in soil. Biodegradation 34, 445–459 (2023). https://doi.org/10.1007/s10532-023-10026-5
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DOI: https://doi.org/10.1007/s10532-023-10026-5