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Monitoring the biodegradability of bisphenol A and its metabolic intermediates by manometric respirometry tests

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Abstract

As a result of its wide usage in the production of polycarbonate plastics and epoxy resins, bisphenol A (BPA) is commonly detected in wastewaters. Recently, BPA became a major concern because its adverse effects as an endocrine disruptor. In this work, the biodegradation kinetics of BPA and its metabolic intermediates 4-hydroxyacetophenone (4HAP), 4-hydroxybenzaldehyde (4HB) and 4-hydroxybenzoic acid (4HBA) by BPA-acclimated activated sludge was studied using manometric respirometry (BOD) tests. For all the tested compounds, BOD curves exhibited two phases. In the first one, a fast increase of the oxygen consumption (OC) due to the active oxidation of the substrate was obtained. Then, when the substrate was exhausted, the endogenous respiration produced a slower increase of OC. A standard Monod-model with biomass growth was used to represent the OC profiles as a function of time. For all the tested compounds, a good agreement between the proposed model and the experimental data was obtained. According to their biodegradation rates, the tested compounds can be ordered as follows: BPA < 4HAP ≪ 4HB < 4HBA. Because the oxidation rate of BPA by BPA-acclimated activated sludge limits the rate of the whole biodegradation pathway, the accumulation of metabolic intermediates 4HAP, 4HB, and 4HBA would be negligible. To calculate the dissolved oxygen (DO) concentration (C) during the BOD tests, the oxygen transfer coefficient (kLa) of the BOD bottles was obtained using the sulfite method. Finally, a simple procedure to calculate the minimum DO concentration (Cmin) based on BOD data was developed. Calculation results demonstrated that under the tested conditions, C values were high enough as not to be the limiting substrate for the microbial growth.

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Authors and Affiliations

  1. Fac. de Ingeniería Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA), UNMdP, Av. Juan B. Justo 4302, CP (B7608FDQ), Mar Del Plata, Buenos Aires, Argentina

    A. M. Ferro Orozco & E. M. Contreras

  2. Fac. de Cs. Exactas Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), UNLP, Calle 115 y 47(s/n), CP (1900), La Plata, Buenos Aires, Argentina

    N. E. Zaritzky

  3. Fac. de Ingeniería, UNLP, Calle 1 y 47, CP(B1900TAG), La Plata, Buenos Aires, Argentina

    N. E. Zaritzky

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  1. A. M. Ferro Orozco
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  2. E. M. Contreras
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  3. N. E. Zaritzky
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Correspondence to E. M. Contreras.

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Ferro Orozco, A.M., Contreras, E.M. & Zaritzky, N.E. Monitoring the biodegradability of bisphenol A and its metabolic intermediates by manometric respirometry tests. Biodegradation 27, 209–221 (2016). https://doi.org/10.1007/s10532-016-9767-4

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