Abstract
A new bacterial strain PH-06 was isolated using enrichment culture technique from river sediment contaminated with 1,4-dioxane, and identified as belonging to the genus Mycobacterium based on 16S rRNA sequencing (Accession No. EU239889). The isolated strain effectively utilized 1,4-dioxane as a sole carbon and energy source and was able to degrade 900 mg/l 1,4-dioxane in minimal salts medium within 15 days. The key degradation products identified were 1,4-dioxane-2-ol and ethylene glycol, produced by monooxygenation. Degradation of 1,4-dioxane and concomitant formation of metabolites were demonstrated by GC/MS analysis using deuterium labeled 1,4-dioxane (1,4-dioxane-d8). In addition to 1,4-dioxane, this bacterium could also transform structural analogues such as 1,3-dioxane, cyclohexane and tetrahydrofuran when pre-grown with 1,4-dioxane as the sole growth substrate. Our results suggest that PH-06 can maintain sustained growth on 1,4-dioxane without any other carbon sources.
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References
Abe A (1999) Distribution of 1,4-dioxane in relation to possible sources in the water environment. Sci Total Environ 227:41–47. doi:10.1016/S0048-9697(99)00003-0
Adams CD, Scanlan PA, Secrist ND (1994) Oxidation and biodegradability enhancement of 1, 4-dioxane using hydrogen peroxide and ozone. Environ Sci Technol 28:1812–1818. doi:10.1021/es00060a010
Bernhardt D, Diekmann H (1991) Degradation of dioxane, tetrahydrofuran and other cyclic ethers by an environmental Rhodococcus strain. Appl Microbiol Biotechnol 36:120–123. doi:10.1007/BF00164711
Boldrin B, Tiehm A, Fritzsche C (1993) Degradation of phenanthrene, fluorene, fluoranthene, and pyrene by a Mycobacterium sp. Appl Environ Microbiol 59:1927–1930
Burback BL, Perry JJ (1993) Biodegradation and biotransformation of groundwater pollutant mixtures by Mycobacterium vaccae. Appl Environ Microbiol 59:1025–1029
DeRosa CT, Wilbur S, Holler J, Richter P, Stevens YW (1996) Health evaluation of 1,4-dioxane. Toxicol Ind Health 12:1–43
Fortnagel P, Harms H, Wittich RM, Krohn S, Meyer H, Sinnwell V, Wilkes H, Francke W (1990) Metabolism of dibenzofuran by Pseudomonas sp. strain HH69 and the mixed culture HH27. Appl Environ Microbiol 56:1148–1156
Hill RR, Jeffs GE, Roberts DR (1997) Photocatalytic degradation of 1, 4-dioxane in aqueous solution. J Photochem Photobiol A 108:55–58. doi:10.1016/S1010-6030(96)04463-2
Khan AA, Kim SJ, Paine DD, Cerniglia CE (2002) Classification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Mycobacterium sp. strain PYR-1, as Mycobacterium vanbaalenii sp. nov. Int J Syst Evol Microbiol 55:593–598
Kim YM, Nam IH, Murugesan K, Schmidt S, Crowley DE, Chang YS (2007) Biodegradation of diphenyl ether and transformation of selected brominated congeners by Sphingomonas sp. PH-07. Appl Microbiol Biotechnol 77:187–194. doi:10.1007/s00253-007-1129-z
Kota S, Borden RC, Barlaz MA (1999) Influence of protozoan grazing on contaminant biodegradation. FEMS Microbiol Ecol 40:179–189. doi:10.1111/j.1574-6941.1999.tb00609.x
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, pp 115–175
Lanigan RS (2000) Addendum to the final report on the safety assessment of polysorbates. Int J Toxicol 19(suppl 2):43–89. doi:10.1080/109158100225033
Mahendra S, Alvarez-Cohen L (2005) Pseudonocardia dioxanivorans sp. nov., a novel actinomycete that grows on 1,4-dioxane. Int J Syst Evol Microbiol 55:593–598. doi:10.1099/ijs.0.63085-0
Mahendra S, Alvarez-Cohen L (2006) Kinetics of 1,4-dioxane biodegradation by monooxygenase-expressing bacteria. Environ Sci Technol 40:5435–5442. doi:10.1021/es060714v
Mahendra S, Petzold CJ, Baidoo EE, Keasling KD, Alvarez-Cohen L (2007) Identification of the intermediates of in vivo oxidation of 1,4-dioxane by monooxygenase-containing bacteria. Environ Sci Technol 41:7330–7336. doi:10.1021/es0705745
Maurino V, Calza P, Minero C, Pelizzetti E, Vincenti M (1997) Light-assisted 1,4-dioxane degradation. Chemosphere 35:2675–2688. doi:10.1016/S0045-6535(97)00322-6
Nakamiya K, Hashimoto S, Ito H, Edmonds JS, Moriat M (2005) Degradation of 1,4-dioxane and cyclic ethers by an isolated fungus. Appl Environ Microbiol 71:1254–1258. doi:10.1128/AEM.71.3.1254-1258.2005
Parales RE, Adamus JE, White N, May HD (1994) Degradation of 1,4-dioxane by an actinomycete in pure culture. Appl Environ Microbiol 60:4527–4530
Park YM, Pyo H, Park SJ, Park SK (2005) Development of the analytical method for 1,4-dioxane in water by liquid-liquid extraction. Anal Chim Acta 548:109–115. doi:10.1016/j.aca.2005.05.057
Popoola AV (1991) Mechanism of the reaction involving the formation of dioxane byproduct during the production of poly (ethylene terephthalate). J Appl Polym Sci 43:1875–1877. doi:10.1002/app.1991.070431011
Roy D, Anagnostu G, Chaphalkar P (1994) Biodegradation of dioxane and diglyme in industrial waste. J Environ Sci Health A 29:129–147
Scalia S (1990) Reversed-phase high-performance liquid chromatographic method for the assay of 1,4-dioxane in sulphated polyoxyethylene alcohol surfactants. J Pharm Biomed 8:867–870. doi:10.1016/0731-7085(90)80134-B
Son HS, Choi SB, Khan E, Zoh KD (2006) Removal of 1,4-dioxane from water using sonication: effect of adding oxidants on the degradation kinetics. Water Res 40(4):692–698. doi:10.1016/j.watres.2005.11.046
Stefan MI, Bolton JR (1998) Mechanisms of the degradation of 1,4-dioxane in dilute aqueous solutions using the UV/hydrogen peroxide process. Environ Sci Technol 32:1588–1595. doi:10.1021/es970633m
Tanabe A, Tsuchida T, Ibaraki T, Kawata K (2006) Impact of 1,4-dioxane from domestic effluent on the Agano and Shinano Rivers, Japan. Bull Environ Contam Toxicol 76:44–51. doi:10.1007/s00128-005-0887-5
Thiemer B, Andreesen JR, Schräder T (2003) Cloning and characterization of a gene cluster involved in tetrahydrofuran degradation in Pseudonocardia sp. strain K1. Arch Microbiol 179:266–277
Vainberg S, McClay K, Masuda H, Root D, Condee C, Zylstra GJ, Steffan RJ (2006) Biodegradation of ether pollutants by Pseudonocardia sp. strain ENV478. Appl Environ Microbiol 72:5218–5224. doi:10.1128/AEM.00160-06
Yu J, Flagan RC, Seinfeld JH (1998) Identification of products containing COOH, OOH, and –C = O in atmospheric oxidation of hydrocarbons. Environ Sci Technol 32:2357–2370. doi:10.1021/es980129x
Zenker MJ, Borden RC, Barlaz MA (2000) Mineralization of 1,4-dioxane in the presence of a structural analog. Biodegradation 11:239–246. doi:10.1023/A:1011156924700
Zenker MJ, Borden RC, Barlaz MA (2003) Occurrence and treatment of 1,4-dioxane in aqueous environments. Environ Eng Sci 20:423–432. doi:10.1089/109287503768335913
Acknowledgments
This research was supported by “Basic Research Project” funded by the Nakdong River Environment Research Center and “The GAIA Project” from the Korea Ministry of Environment.
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Kim, YM., Jeon, JR., Murugesan, K. et al. Biodegradation of 1,4-dioxane and transformation of related cyclic compounds by a newly isolated Mycobacterium sp. PH-06. Biodegradation 20, 511–519 (2009). https://doi.org/10.1007/s10532-008-9240-0
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DOI: https://doi.org/10.1007/s10532-008-9240-0