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Hydrogen production and anaerobic decolorization of wastewater containing Reactive Blue 4 by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa

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Abstract

Anaerobic biodegradability of wastewater (3,000 mg CODcr/l) containing 300 mg/l Reactive Blue 4, with different co-substrates, glucose, butyrate and propionate by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa, concomitantly with hydrogen production was investigated at 35°C. The accumulative hydrogen production at 3,067 mg CODcr/l was obtained after 7 days of incubation with glucose, sludge, the bacterial consortium. The volatile fatty acids, residual glucose and the total organic carbon were correlated to hydrogen obtained. Interestingly, the bacterial consortium possess decolorization ability showing approximately 24% dye removal after 24 h incubation using glucose as a co-substrate, which was about two and eight times those of butyrate (10%), propionate (12%) and control (3%), respectively. RB4 decolorization occurred through acidogenesis, as high volatile fatty acids but low methane was detected. The bacterial consortium will be the bacterial strains of interest for further decolorization and hydrogen production of industrial waste water.

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Acknowledgments

This research was supported by JSPS-NRCT (Microbial Resources Program) and Thailand Research Fund.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, 10110, Thailand

    Ramida Yuwadee Watanapokasin, Supawadee Sukseree & Aungkana Krajarng

  2. Graduate School of Science and Engineering, Yamaguchi University, Tokiwadai 2-26-1, Yamaguchi, 755-8611, Japan

    Anantabhathra Boonyakamol & Tsuyoshi Imai

  3. Department of Biochemistry, Faculty of Science, Rangsit University, Muangake, Pathumthani, 12000, Thailand

    Thanet Sophonnithiprasert

  4. Department of Biological Science, Faculty of Science, Ubonratchathani University, Warinchamrab, Ubonratchathani, 34190, Thailand

    Sungwan Kanso

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  1. Ramida Yuwadee Watanapokasin
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  2. Anantabhathra Boonyakamol
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Correspondence to Ramida Yuwadee Watanapokasin.

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Watanapokasin, R.Y., Boonyakamol, A., Sukseree, S. et al. Hydrogen production and anaerobic decolorization of wastewater containing Reactive Blue 4 by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa . Biodegradation 20, 411–418 (2009). https://doi.org/10.1007/s10532-008-9232-0

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  • DOI: https://doi.org/10.1007/s10532-008-9232-0

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