Skip to main content

Advertisement

SpringerLink
Log in

Genetic Variation in Three Endangered Species of Encholirium (Bromeliaceae) from Cadeia do Espinhaço, Brazil, Setected using RAPD Markers

  • Published:
Biodiversity & Conservation Aims and scope Submit manuscript

Abstract

Encholirium is a Brazilian genus of Bromeliaceae that occurs exclusively in rocky landscapes. This work aimed to generate basic information for the conservation of three Encholirium species that are endemic to the rocky mountains of Cadeia do Espinhaço, employing population genetic analyses. E. pedicellatum and E. biflorum have only one very small population each, both occurring in unprotected, private land sites, being critically endangered. E. subsecundum is more widespread, and some of its populations dwell in protected areas. Five Random Amplified Polymorphic DNA (RAPD) primers generated approximately 60 polymorphic bands for each species. This technique demonstrated the presence of a single RAPD profile for every individual sampled (except for one clone found in E. biflorum). High levels of genetic variability were not expected, due to the clonal habit of the plants and small size of the populations. Populations of E. biflorum and E. pedicellatum presented, respectively, 16.06% (Φ st  = 0.16, p<0.001) and 8.44% (Φ st  = 0.08, p<0.001) of the total genetic diversity attributable to genetic differences among groups within the populations. In E. subsecundum, 14.52% (Φ st  = 0.15, p<0.001) of the total genetic diversity was found among populations. Estimates of the Shannon’s Diversity Index provided similar results. These results are valuable for the development of conservation strategies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allnutt T.R., Newton A.C., Premoli A. and Lara A. (2003). Genetic variation in the threatened South American conifer Pilgerodendron uviferum (Cupressaceae), detected using RAPD markers. Biol. Conserv. 114: 245–253

    Article  Google Scholar 

  • Ayres D.R. and Ryan F.J. (1999). Genetic diversity and structure of the narrow endemic Wyethia reticulata its congener W. bolanderi (Asteraceae) using RAPD and allozyme techniques. Am. J. Bot. 86: 344–353

    Article  PubMed  Google Scholar 

  • Bekessy S.A., Allnutt T.R., Premoli A.C., Lara A., Ennos R.A., Burgman M.A., Cortes M. and Newton A.C. (2002). Genetic variation in the vulnerable and endemic Monkey Puzzle treedetected using RAPDs. Heredity 88: 243–249

    Article  PubMed  CAS  Google Scholar 

  • Chung M.G., Chung J.M. and Epperson B.K. (1999). Spatial genetic structure of allozyme polymorphisms within populations of Rhus trichocarpa (Anacardiaceae). Silvae Genetica 48: 223–227

    Google Scholar 

  • Coelho A.S.G. 2000. Dboot: avaliação dos erros associados a estimativas de distâncias/ similaridades genéticas através do procedimento de bootstrap com número variável de marcadores (software).[Dboot: Assessment of Errors Associated to Estimates of Genetic Distances/Similarities by Bootstrapping with a Variable Number of Markers (software)]. Laboratório de Genética Vegetal, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Brazil.

  • Coelho A.S.G. 2003. Bood-P: avaliação de dendrogramas baseados em estimativas de distâncias/ similaridades genéticas através do procedimento de bootstrap (software). [Bood-P: Assessment of Dendrograms based on Estimates of Genetic Distances/Similarities by Bootstrapping (software)]. Laboratório de Genética Vegetal, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia.

  • Costa C.M.R., Hermann G., Martins C.S., Lins L.V. and Lamas I.R. (1998). Biodiversidade em Minas Gerais: um Atlas para sua conservação. Fundação Biodiversitas, Belo Horizonte

    Google Scholar 

  • Doyle J.J. and Doyle J.L. (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13–15

    Google Scholar 

  • Ellstrand N.C. and Elam D.R. (1993). Population genetic consequences of small population size: implications for plant conservation. Annu. Rev. Ecol. Syst. 24: 217–242

    Article  Google Scholar 

  • Esselman E.J., Jianqiang L., Crawford D.J., Winduss J.L. and Wolfe A.D. (1999). Clonal diversity in the rare Calamagrostis porteri spp insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Mol. Ecol. 8: 443–451

    Article  CAS  Google Scholar 

  • Excoffier L., Smouse P.E. and Quattro J.M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes – application to human mitochondrial – DNA restriction data. Genetics 131: 479–491

    PubMed  CAS  Google Scholar 

  • Fischer M., Husi R., Prati D., Peintinger M., van Kleunen M. and Schmid B. (2000). RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae). Am. J. Bot. 87: 1128–1137

    Article  PubMed  Google Scholar 

  • Forzza R.C. (2005). Revisão taxonômica de Encholirium Mart. ex. Schult. & Schult. f. (Pitcairnioideae – Bromeliaceae). Boletim de Botânica da Universidade de São Paulo 23: 1–60

    Google Scholar 

  • Forzza R.C., Christianini A.V. and Wanderley M.G.L. (2003). Encholirium (Pitcairnioideae – Bromeliaceae): conhecimento atual e sugestões para conservação. Vidalia 1: 7–20

    Google Scholar 

  • Gentry A.H. (1986). Endemism in tropical versus temperate plant communities. In: Soulé, M.E. (eds) Conservation Biology – The Science of Scarcity and Diversity, Sinauer Associates, Sunderland Massachusetts 153–181

    Google Scholar 

  • Giulietti A.M., Pirani J.R. and Harley R.M. (1997). Espinhaço range region. In: Davis, S.D., Heywood, V.H., Herrera-Macbryde, O., Villa-Lobos, J., and Hamilton, A. (eds) Centres of Plant Diversity: A Guide and Strategy for Their Conservation. Volume 3: The Americas, IUCN Publications Unit, Cambridge 397–404

    Google Scholar 

  • Hamrick J.L. and Godt J.W. (1989). Allozyme diversity in plant species. In: Brown, A.H.D., Clegg, M.T. and Kahler, A.L. (eds) Plant Population Genetics, Breeding, and Genetic Resources, Sinauer Associates, Sunderland, Massachusetts 43–63

    Google Scholar 

  • Holsinger K.E. and Gottlieb L.D. (1991). Conservation of rare and endangered plants: principles and prospects. In: Falk, D.A. and Holsinger, K.E. (eds) Genetics and Conservation of Rare Plants, Oxford, Oxford University Press 195–223

    Google Scholar 

  • Huff D.R., Peakall R. and Smouse P.E. (1993). RAPD variation within and among natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.) Engelm.]. Theor. Appl. Genet. 86: 927–934

    Article  CAS  Google Scholar 

  • James E.A. and Ashburner G.R. (1997). Intraspecific variation in Astelia australiana (Liliaceae) and implications for the conservation of this Australian species. Biol. Conserv. 82: 253–261

    Article  Google Scholar 

  • Kreher S.A., Foré S.A. and Collins B.S. (2000). Genetic variation within and among patches of the clonal species Vaccinium stamineum L. Mol. Ecol. 9: 1247–1252

    Article  PubMed  CAS  Google Scholar 

  • Lacerda D.R., Acedo M.D.P., Lemos-Filho J.P. and Lovato M.B. (2001). Genetic diversity and structure of natural populations of Plathymenia reticulata (Mimosoideae), a tropical tree from Brazilian Cerrado. Mol. Ecol. 10: 1143–1152

    Article  PubMed  CAS  Google Scholar 

  • Lewontin R.C. (1972). The Apportionment of Human Diversity. Evol. Biol. 6: 381–494

    Google Scholar 

  • Maki M. and Horie S. (1999). Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fischerianum var. molle than in the widespread conspecific C. fischerianum var. fischerianum (Caryophyllaceae). Mol. Ecol. 8: 145–150

    Article  CAS  Google Scholar 

  • Persson H.A. and Gustavsson B.A. (2001). The extent of clonality and genetic diversity in lingonberry (Vaccinium vitis- idaea L.) revealed by RAPDs and leaf-shape analysis. Mol. Ecol. 10: 1385–1397

    Article  PubMed  CAS  Google Scholar 

  • Rohlf F.J. (1989). NTSYS-Pc: Numerical Taxonomy and Multivariate Analysis System. Exerter Publisher, New York, 210

    Google Scholar 

  • Sazima I., Vogel S. and Sazima M. (1989). Bat pollination of Encholirium glazioviia terrestrial bromeliad. Plant Syst. Evol. 168: 167–179

    Article  Google Scholar 

  • Schneider S., Roessli D. and Excoffier L. (2000). Arlequin ver. 2000: A Software for Population Data Analysis (software). Genetic and Biometry Laboratory, University of Geneva, Geneva

    Google Scholar 

  • Tansley S.A. and Brown C.R. (2000). RAPD variation in the rare and endangered Leucadendron elimense (Proteaceae): implications for their conservation. Biol. Conserv. 95: 39–48

    Article  Google Scholar 

  • Welsh J. and McClelland M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 303–306

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Genética, Escola Superior de Agricultura ‘Luiz de Queiroz’ (ESALQ), Universidade de São Paulo, CP 83, 13400-970, Piracicaba, São Paulo, Brazil

    Marcelo Mattos Cavallari, Elizabeth Ann Veasey, Maria Imaculada Zucchi & Giancarlo Conde Xavier Oliveira

  2. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ), Rua Pacheco Leão 915, 22460-030, Rio de Janeiro, Brazil

    Rafaela Campostrini Forzza

Authors
  1. Marcelo Mattos Cavallari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rafaela Campostrini Forzza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elizabeth Ann Veasey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Imaculada Zucchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Giancarlo Conde Xavier Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcelo Mattos Cavallari.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cavallari, M.M., Forzza, R.C., Veasey, E.A. et al. Genetic Variation in Three Endangered Species of Encholirium (Bromeliaceae) from Cadeia do Espinhaço, Brazil, Setected using RAPD Markers. Biodivers Conserv 15, 4357–4373 (2006). https://doi.org/10.1007/s10531-005-3741-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10531-005-3741-5

Keywords

Search

Navigation