Abstract
Objective
Salidroside is an important plant-derived aromatic compound with diverse biological properties. The main objective of this study was to synthesize salidroside from tyrosol using UDP-glucosyltransferase (UGT) with in situ regeneration of UDP-glucose (UDPG).
Results
The UDP-glucosyltransferase 85A1 (UGT85A1) from Arabidopsis thaliana, which showed high activity and regioselectivity towards tyrosol, was selected for the production of salidroside. Then, an in vitro cascade reaction for in situ regeneration of UDPG was constructed by coupling UGT85A1 to sucrose synthase from Glycine max (GmSuSy). The optimal UGT85A1-GmSuSy activity ratio of 1:2 was determined to balance the efficiency of salidroside production and UDP-glucose regeneration. Different cascade reaction conditions for salidroside production were also determined. Under the optimized condition, salidroside was produced at a titer of 6.0 g/L with a corresponding molar conversion of 99.6% and a specific productivity of 199.1 mg/L/h in a continuous feeding reactor.
Conclusion
This is the highest salidroside titer ever reported so far using biocatalytic approach.
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Acknowledgements
This work was funded by the Open Fund of Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources (TCMRPSU-2022-03), the Natural Science Foundation of Higher Education Institutions of Anhui Province (2023AH052649), the Fund of Generic Technology Research center for Anhui Traditional Chinese Medicine Industry (AHTCMGTRC-2023-09), the National Natural Science Foundation of China (32201979) and the University Research Project of Anhui Province (2022AH051682).
Funding
Open Fund of Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources (TCMRPSU-2022-03), Natural Science Foundation of Higher Education Institutions of Anhui Province (2023AH052649), Fund of Generic Technology Research center for Anhui Traditional Chinese Medicine Industry (AHTCMGTRC-2023-09), National Natural Science Foundation of China (32201979), and University Research Project of Anhui Province (2022AH051682).
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Li, G., Xu, Q., Hu, N. et al. Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction. Biotechnol Lett 46, 173–181 (2024). https://doi.org/10.1007/s10529-023-03453-0
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DOI: https://doi.org/10.1007/s10529-023-03453-0