Abstract
Hfq is an RNA-binding protein, its main function is to participate in post-transcriptional regulation of bacteria and regulate small regulatory RNA (sRNA) and messenger RNA (mRNA) stability, but the Hfq function of Bacillus subtilis (B. subtilis) has not been fully explained. In this study, we used the strains of B. subtilis168 (BS168), BS168Δhfq and BS168Δhfq-C to explore the effects of Hfq on the glucose utilization, biofilm formation and quorum sensing (QS) system of B. subtilis. The results showed that the knockout of hfq resulted in growth defects when bacteria were cultured in the Luria–Bertani (LB) medium, but we did not observe the same effects in Nitrogen medium (NM) and Inorganic Salt-free medium (ISM). We further found that the growth of strains under different glucose concentrations was also different, which was related to the expression of CcpA. Interestingly, the hfq mutant showed increased resistance to a high-glucose environment. Furthermore, the biofilm and extracellular poly saccharides (EPS) formation of BS168Δhfq decreased significantly. At the same time, changes were observed in the morphology of the biofilm, such as larger intercellular space of the biofilm and thinner edge. The qRT-PCR results confirmed that the hfq knockout caused significant up-regulation or down-regulation of gene expression in QS system, and down-regulated genes were involved in the positive regulation of biofilm formation. Taken together, we demonstrated that Hfq plays a vital role in glucose utilization, biofilm formation and QS of B. subtilis, which provides a new perspective for subsequent related research.
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The datasets generated during the current study are available from the corresponding author on reasonable request.
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This work was supported by Chengdu Giant Panda Breeding Research Foundation (CPF2015-09, CPF2017-12) (Chengdu, China).
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All authors contributed to the study conception and design. MYD and XTY conducted the design study; MYD conducted the study and wrote the manuscript; LJL and ZYZ analyzed the data; ZJZ, HFL, HLF, XPM, SZC, LHS revised the important intellectual content, and GNP gave final approval to the submitted version.
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Dong, M., Yang, X., Liu, L. et al. Role of Hfq in glucose utilization, biofilm formation and quorum sensing system in Bacillus subtilis. Biotechnol Lett 44, 845–855 (2022). https://doi.org/10.1007/s10529-022-03262-x
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DOI: https://doi.org/10.1007/s10529-022-03262-x