Abstract
Objectives
To immobilize Candida rugosa lipase in Accurel MP 1000 (CRL-AMP) by physical adsorption in organic medium and apply in the synthesis of wax esters dodecanoyl octadecanoate 1 and hexadecanoyl octadecanoate 2 in a heptane medium, as well as evaluating the stability and recyclability of CRL-AMP in six reaction cycles.
Results
The specific activity (Asp) for CRL-AMP was 200 ± 20 U mg−1. Its catalytic activity was 1300 ± 100 U g−1. CRL-AMP was used in the synthesis of esters in heptane medium with a 1:1 acid:alcohol molar ratio at 45 °C and 200 rpm. In synthesis 1, conversion was 62.5 ± 3.9% in 30 min at 10% m v−1 and 56.9 ± 2.8% in 54 min at 5% m v−1; while in synthesis 2, conversion was 79.0 ± 3.9% in 24 min at 10% m v−1, and 46.0 ± 2.4% in 54 min at 5% m v−1. Reuse tests after six consecutive cycles of reaction showed that the biocatalyst retained approximately 50% of its original activity for both reaction systems.
Conclusions
CRL-AMP showed a high potential in the production of wax esters, since it started from low enzymatic load and high specific activities and conversions were obtained, in addition to allowing an increase in stability and recyclability of the prepared biocatalyst.
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The Coordination for the Improvement of Higher Level Personnel (CAPES) for financial support and the State University of Santa Cruz (UESC) for administrative and technical support.
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de Menezes, L.H.S., do Espírito Santo, E.L., dos Santos, M.M.O. et al. Candida rugosa lipase immobilized on hydrophobic support Accurel MP 1000 in the synthesis of emollient esters. Biotechnol Lett 44, 89–99 (2022). https://doi.org/10.1007/s10529-021-03196-w
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DOI: https://doi.org/10.1007/s10529-021-03196-w