Abstract
Objectives
This study reports the identification of a new bacterial azoreductase (AzoR) from Klebsiella oxytoca GS-4-08, its heterologous production in Escherichia coli and the decolorization of azo dyes.
Results
The AzoR, as a flavin-free, oxygen-insensitive enzyme, has a molecular mass of 22 kDa and a high substrate specificity for methyl red (MR). In the presence of 5 mM of NADH, the enzyme activity for decolorization of 50 mg l−1 of MR reached 5.61 μmol min−1 mg protein−1. Double-reciprocal plots indicated that the NADH and MR reductions proceed by ping-pong mechanism. The calculated Vm was 8.17 μM min−1 mg protein−1, and the AzoR retained over 75% of relative activity under temperature range from 20 to 50 °C.
Conclusion
This study for the first time identified an AzoR from Klebsiella oxytoca strain, which enabled efficient degradation of azo dyes by strain GS-4-08.
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (No. 51778298) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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Hua, Jq., Yu, L. Cloning and characterization of a Flavin-free oxygen-insensitive azoreductase from Klebsiella oxytoca GS-4-08. Biotechnol Lett 41, 371–378 (2019). https://doi.org/10.1007/s10529-019-02647-9
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DOI: https://doi.org/10.1007/s10529-019-02647-9