Abstract
Objective
To isolate cyclic (1 → 3, 1 → 6)-β-glucan from Bradyrhizobium japonicum MTCC120, to characterize its structure and to study its biological activities.
Results
The degree of polymerization of cyclic (1 → 3, 1 → 6)-β-glucan varied between 10 and 13 and with substituents acetyl, succinyl and phosphocholine. The cyclic glucans showed bimodal particle size distribution, with hydrodynamic diameters of 1.92 and 231 nm corresponding to monomeric and aggregated cyclic glucans, respectively. SEM and TEM images showed that the glucans formed aggregates of nanorods. The glucans were biocompatible, exhibited good antioxidant activity and had the abilities to bind to Aniline Blue dye to form a fluorescence complex which was concentration dependent.
Conclusion
The glucans isolated are cyclic and have good antioxidant activities, hence have potential application in food and pharmaceutical industries. Their dye binding ability could be exploited in medical imaging to reduce the cytotoxicity of the dyes.
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Acknowledgments
We thank Department of Biotechnology, Government of India for financial support. Department of metallurgical and materials engineering, DST unit of nanoscience and thematic unit of Excellence, Department of chemistry and Sophisticated analytical Instrument facility (SAIF), IIT-Madras, for their analytical instrument facilities. Authors also thank Mr. Rakesh Pandeet Nankar, for help in cell culture studies.
Supporting Information
Supplementary Figure 1—TLC of cyclic (1 → 3, 1 → 6)-β-glucan.
Supplementary Figure 2—Compositional analysis of glucan.
Supplementary Figure 3—FTIR spectrum of cyclic (1 → 3, 1 → 6)-β-glucan.
Supplementary Figure 4—1H- 1H COSY NMR spectrum of cyclic (1 → 3, 1 → 6)-β-glucan.
Supplementary Figure 5—(a) SEM and (b) TEM images of cyclic β-glucan. (c) Particle size analysis of cyclic β-glucan.
Supplementary Figure 6—(a) Fluorescence assay of cyclic β-glucan and Aniline Blue dye complex. Aniline Blue bound to glucan powder (c) Fluorescence microscopic image of Aniline Blue bound cyclic β-glucan.
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Nair, A.V., Gummadi, S.N. & Doble, M. Characterization and biological activities of cyclic (1 → 3, 1 → 6)-β-glucans from Bradyrhizobium japonicum . Biotechnol Lett 38, 1519–1525 (2016). https://doi.org/10.1007/s10529-016-2122-3
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DOI: https://doi.org/10.1007/s10529-016-2122-3