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Expression and characterization of a cold-adapted, thermotolerant and denaturant-stable GH5 endoglucanase Celal_2753 that withstands boiling from the psychrophilic bacterium Cellulophaga algicola IC166T

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Abstract

Objectives

To characterize a novel endoglucanase, Celal_2753, from the psychrophilic bacterium Cellulophaga algicola IC166T.

Results

Celal_2753 was purified to homogeneity with a yield of 81 % and with a molecular weight of 40 kDa on SDS-PAGE. It had maximum hydrolytic activity towards carboxymethyl cellulose at 40 °C and pH 6. It showed 33 % of the maximum activity at 10 ºC. Its activity increased to 272–316 % in the presence of 0.25–2 M NaCl and KCl at 40 °C. Celal_2753 was stable in the presence of 10 % (v/v) Tween 20, 10 % (v/v) Triton X-100, 16 mM SDS, 6 M urea or 2 M guanidine hydrochloride. Celal_2753 that had been boiled for 5 min recovered 55 % of its initial activity by incubating at 30 °C for 60 min.

Conclusion

Because of its cold-adapted, thermotolerant and denaturant-stable properties, endoglucanase Celal_2753 is promising in detergent industry and bioethanol production.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31000361), Natural Science Foundation of Shandong Province (ZR2015CM024) and the Fundamental Research Funds for the Central Universities (201013008).

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Authors and Affiliations

  1. Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao, 266003, China

    Ying Wang, Wengong Yu & Feng Han

  2. Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology, 5 Yushan Road, Qingdao, 266003, China

    Ying Wang, Wengong Yu & Feng Han

  3. School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Ying Wang, Wengong Yu & Feng Han

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  1. Ying Wang
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  2. Wengong Yu
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  3. Feng Han
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Correspondence to Feng Han.

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Wang, Y., Yu, W. & Han, F. Expression and characterization of a cold-adapted, thermotolerant and denaturant-stable GH5 endoglucanase Celal_2753 that withstands boiling from the psychrophilic bacterium Cellulophaga algicola IC166T . Biotechnol Lett 38, 285–290 (2016). https://doi.org/10.1007/s10529-015-1971-5

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  • DOI: https://doi.org/10.1007/s10529-015-1971-5

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