Abstract
Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.
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Abbreviations
- AN:
-
Acid number
- M(KOH) :
-
Molar mass of KOH
- c(KOH) :
-
Concentration of KOH solution
- V(KOH) :
-
Volume KOH solution applied during titration
- m(oil) :
-
Mass of the oil sample
- M(FFA) :
-
Average molecular weight of the free fatty acids (with regard to the fatty acid composition of rapeseed oil)
- ρ(oil) :
-
Density of rapeseed oil
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Acknowledgments
The present work was funded by the Federal Ministry of Education and Research in line with a cooperation program “Programme Inter Carnot Fraunhofer (PICF 2010)”.
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von der Haar, D., Stäbler, A., Wichmann, R. et al. Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases. Biotechnol Lett 37, 169–174 (2015). https://doi.org/10.1007/s10529-014-1668-1
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DOI: https://doi.org/10.1007/s10529-014-1668-1