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Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers

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Abstract

An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbP CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbP CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbM) CO17-1A. Similar to mAbM CO17-1A, treatment with mAbP CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbP CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbM CO17-1A. These results suggest that mAbP CO17-1A is as effective on anti-cancer activity as mAbM CO17-1A.

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Acknowledgments

This research was supported by a Grant (Code# PJ009419) from the Korean Rural Development Administration.

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Authors and Affiliations

  1. Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Jae-Sung Ryu, Ju-Taek Lee, Malg-Um Lim, Mi-Ran Hwang & Young-Kug Choo

  2. Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-853, Korea

    Kyung-A Hwang

  3. Department of Pharmaceutical Engineering, Konyang University, Nonsan, 320-711, Korea

    Young-Ho Cho

  4. Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Heukseok-ro 84, Seoul, 156-756, Korea

    Jeong-Hwan Lee & Kisung Ko

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  1. Jae-Sung Ryu
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Correspondence to Kisung Ko or Young-Kug Choo.

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Ryu, JS., Lee, JT., Lim, MU. et al. Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers. Biotechnol Lett 35, 2031–2038 (2013). https://doi.org/10.1007/s10529-013-1318-z

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  • DOI: https://doi.org/10.1007/s10529-013-1318-z

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