Abstract
To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l−1 h−1) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l−1 h−1) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources.
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Acknowledgments
We thank Feng-Yan Bai in Institute of Microbiology, China, for providing the S. cerevisiae strain JZ1C and Kjeld Olesen at Carlsberg laboratory, Denmark, for providing the vector pYC230. This study was supported by Chinese Academy of Sciences (KSCX2-EW-J-10).
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Yuan, B., Wang, SA. & Li, FL. Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources. Biotechnol Lett 35, 1589–1592 (2013). https://doi.org/10.1007/s10529-013-1251-1
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DOI: https://doi.org/10.1007/s10529-013-1251-1