Skip to main content
SpringerLink
Log in

Mapping of the quantitative trait locus (QTL) conferring partial resistance to rice leaf blast disease

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Malaysian rice, Pongsu Seribu 2, has wide-spectrum resistance against blast disease. Chromosomal locations conferring quantitative resistance were detected by linkage mapping with SSRs and quantitative trait locus (QTL) analysis. For the mapping population, 188 F3 families were derived from a cross between the susceptible cultivar, Mahsuri, and a resistant variety, Pongsu Seribu 2. Partial resistance to leaf blast in the mapping population was assessed. A linkage map covering ten chromosomes and consisting of 63 SSR markers was constructed. 13 QTLs, including 6 putative and 7 putative QTLs, were detected on chromosomes 1, 2, 3, 5, 6, 10, 11 and 12. The resulting phenotypic variation due to a single QTL ranged from 2 to 13 %. These QTLs accounted for approx. 80 % of the total phenotypic variation within the F3 population. Therefore, partial resistance to blast in Pongsu Seribu 2 is due to combined effects of multiple loci with major and minor effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmed NU, Park JI, Jung HJ, Seo MS, Kumar TS, Lee IH, Nou IS (2012) Identification and characterization of stress resistance related genes of Brassica rapa. Biotechnol Lett 34:979–987. doi:10.1007/s10529-012-0860-4

    Article  PubMed  CAS  Google Scholar 

  • Ashkani S, Rafii MY, Sariah M, Siti Nor Akmar A, Rusli I, Abdul Rahim H, Latif MA (2011) Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa L.). Gen Mol Res 10:1345–1355

    Article  CAS  Google Scholar 

  • Ashkani S, Rafii MY, Rusli I, Meon S, Siti Nor Akmar A, Abdul Rahim H, Latif MA (2012) SSRs for marker assisted selection for blast resistance in rice (Oryza sativae L.). Plant Mol Biol Reprod 30:79–86

    Article  Google Scholar 

  • Chen H (2001) Population structure of Pyricularia grisea from central and southern China and comparative mapping of QTL for blast and bacterial blight resistance in rice and barley (in Chinese). Dissertation, Huazhong Agriculture University, Wuhan

    Google Scholar 

  • Cho YC, Kwon SW, Suh JP, Kim JJ, Lee JH, Roh JH, Oh MK, Kim MK, Ahn SN, Koh HJ (2008) QTL identification and confirmation of field resistance to leaf blast in temperate japonica rice (Oryza sativa L.). J Crop Sci Biotechnol 11:269–276

    Google Scholar 

  • Correa-Victoria FJ, Zeigler RS (1993) Pathogenic variability in Pyricularia grisae at a rice blast “hot spot” breeding site in Eastern Colombia. Plant Dis 77:1029–1035

    Article  Google Scholar 

  • Couch BC, Kohn LM (2002) A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea. Mycologia 94:683–693

    Article  PubMed  CAS  Google Scholar 

  • Filippi MC, Prahbu AS (2001) Phenotypic virulence analysis of Pyricularia grisea isolates from Brazilian upland rice cultivars. Pesqui Agropecu Bras 36:27–35

    Article  Google Scholar 

  • Fjellstrom R, Conaway-Bormans CA, McClung A, Marchetti MA, Shank AR, Park WD (2004) Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisae pathotypes. Crop Sci 44:1790–1798

    Article  CAS  Google Scholar 

  • Haley CS, Knott SA (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324

    Article  PubMed  CAS  Google Scholar 

  • IRRI (1996) Standard evaluation system for rice, 4th edn. The international network for genetic evaluation of rice, Genetic Resources Center. International Rice Research Institute, Manila, 52

  • Koide Y, Kobayashi N, Xu D, Fukuta Y (2009) Resistance genes and selection DNA markers for blast disease in rice (Oryza sativa L.). JARQ 43:255–280

    Article  CAS  Google Scholar 

  • Koizumi S (2007) Durability of resistance to rice blast disease. A differential system for blast resistance for stable rice production environment. JIRCAS working report, 1–10

  • Kosambi DD (1994) The estimation of map distance from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Latif MA, Rafii MY, Rahman MM, Talukdar MRB (2011) Microsatellite and minisatellite markers based DNA fingerprinting and genetic diversity of blast and ufra resistant genotypes. CR Biologies 334:282–289

    Article  CAS  Google Scholar 

  • Lopez-Gerena L (2006) Mapping QTL controlling durable resistance to rice blast in cultivar Oryza llanos 5. Kansas State University, Kansas

    Google Scholar 

  • Mackill D, Bonman J (1992) Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82:746–749

    Article  Google Scholar 

  • Manly KF, Cudmore RH Jr, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932

    Article  PubMed  CAS  Google Scholar 

  • McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13

    Google Scholar 

  • McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.) DNA Res 9:199–207

    Article  PubMed  CAS  Google Scholar 

  • Nelson JC (1997) QGENE: software for maker-based genomics analysis and breeding. Mol Breed 3:239–245

    Article  CAS  Google Scholar 

  • Pan QH, Wang L, Ikehashi H, Tanisaka T (1996) Identification of a new blast resistance gene in the indica rice cultivar Kasalath using Japanese differential cultivars and isozyme markers. Phytopathology 86:1071–1075

    Article  Google Scholar 

  • Pessoa-Filho M, Beló A, Alcochete AAN, Rangel PHN, Ferreira ME (2007) A set of multiplex panels of microsatellite markers for rapid molecular characterization of rice accessions. BMC Plant Biol 7:23

    Article  PubMed  Google Scholar 

  • Prabhu AS, Castro EM, Araújo LG, Berni RF (2003) Resistance spectra of six elite breeding lines of upland rice to Pyricularia grisea. Pesqui Agropecu Bras 38:203–210

    Article  Google Scholar 

  • Rahim H (2010) Genetic studies on blast disease (Magnaporthe grisea) resistance in Malaysian rice. Universiti Kebangsaan, Selangor

    Google Scholar 

  • Sallaud C, Lorieux M, Roumen E, Tharreau D, Berruyer R, Svestasrani P, Garsmeur O, Ghesquiere A, Notteghem JL (2003) Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theor Appl Genet 106:794–803

    PubMed  CAS  Google Scholar 

  • Sharma TR, Madhav MS, Singh BK, Shanker P, Jana TK, Dalal V, Pandit A, Singh A, Gaikwad K, Upreti HC, Singh NK (2005) High-resolution mapping, cloning and molecular characterization of the Pi-k (h) gene of rice, which confers resistance to Magnaporthe grisea. Mol Genet Genomics 274:1–10

    Article  Google Scholar 

  • Sirithunya P, Tragoonrung S, Vanavichit A, Pa-In N, Vongsaprom C, Toojinda T (2002) Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (Oryza sativa). DNA Res 9:79–88

    Article  PubMed  CAS  Google Scholar 

  • Tabien RE, Li Z, Paterson AH, Marchetti MA, Stansel JW, Pinson SRM, Park WD (2000) Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’ and evaluation of their combinatorial effect for field resistance. Theor Appl Genet 101:1215–1225

    Article  CAS  Google Scholar 

  • Tabien R, Li Z, Paterson AH, Marchetti MA, Stansel JW, Pinson SRM (2002) Mapping QTLs for field resistance to the rice blast pathogen and evaluating their individual and combined utility in improved varieties. Theor Appl Genet 105:313–324

    Article  PubMed  CAS  Google Scholar 

  • Talbot NJ (2003) On the trail of a cereal killer: exploring the biology of Magnaporthe grisea. Annu Rev Microbiol 57:177–202

    Article  PubMed  CAS  Google Scholar 

  • Wang G, Ding X, Yuan M, Qiu D, Li X, Xu C, Wang S (2006) Dual function of rice OsDR8 gene in disease resistance and thiamine accumulation. Plant Mol Biol 60:437–449

    Article  PubMed  CAS  Google Scholar 

  • Zenbayashi K, Ashizawa T, Tani T, Koizumi S (2002) Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32. Theor Appl Genet 104:547–552

    Article  PubMed  CAS  Google Scholar 

  • Zhou JH, Wang JL, Xu JC, Lei CL, Ling ZZ (2004) Identification and mapping of a rice blast resistance gene Pi-g(t) in the cultivar Guang changzhan. Plant Pathol 53:191–196

    Article  CAS  Google Scholar 

  • Zhu M, Wang L, Pan QH (2004) Identification and characterization of a new blast resistance gene located on rice chromosome 1 through linkage and differential analyses. Phytopathology 94:515–519

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research activity was funded by the Long term Research Grants Scheme (LRGS), Rice Food Security Project, Ministry of Higher Education, Malaysia. We would like to express our sincere thanks to the Malaysian Nuclear Agency for providing research facilities. We are also grateful to the Malaysian Rice Research Centre, MARDI, for supplying pathogen isolates and providing greenhouse facilities.

Author information

Authors and Affiliations

  1. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    S. Ashkani, M. Y. Rafii & M. A. Latif

  2. Department of Agronomy and Plant Breeding, Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran

    S. Ashkani

  3. Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. Y. Rafii

  4. Agrotechnology and Bioscience Division, Malaysian Nuclear Agency, Bangi, Kajang, Selangor, Malaysia

    H. A. Rahim

  5. Bangladesh Rice Research Institute (BRRI), Gazipur, 1701, Bangladesh

    M. A. Latif

Authors
  1. S. Ashkani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Y. Rafii
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. A. Rahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. A. Latif
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Y. Rafii.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 70 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ashkani, S., Rafii, M.Y., Rahim, H.A. et al. Mapping of the quantitative trait locus (QTL) conferring partial resistance to rice leaf blast disease. Biotechnol Lett 35, 799–810 (2013). https://doi.org/10.1007/s10529-012-1130-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-012-1130-1

Keywords

Search

Navigation