Abstract
Human mesenchymal stem cells (MSC) were seeded onto the inner surface of a tubular silicon construct and, after 24 h, were exposed to a shearing stress of either 2.5 or 10 dyne/cm2 for 1 day. The fluid contained endothelial growth factors in both cases. Morphological changes and cytoskeletal rearrangements were observed in the stimulated cells. Immunofluorescence staining showed that low (2.5 dyne/cm2) and high shear stress (10 dyne/cm2) resulted in the expression of von Willebrand factor (vWF) and calponin, respectively. At low shear stress, CD31 (PECAM-1) was significantly expressed whereas vWF and KDR expression was only slightly higher than those under 10 dyne/cm2. All three markers related to smooth muscle cells (myocardin, myosin heavy chain, and SM‐22α) had significantly higher expression under shear stress of 10 dyne/cm2 compared with a 2.5 dyne/cm2, even in endothelial growth medium. Shear stress plays a critical role in regulating MSC differentiation and must be considered for bioengineered blood vessels.
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This work was supported by the grants of Technology Innovation Program (10038667, Ministry of Knowledge Economy, ROK) and Priority Research Centers Program(2010-0020224, the Ministry of Education, Science and Technology).
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Kim, D.H., Heo, SJ., Kim, SH. et al. Shear stress magnitude is critical in regulating the differentiation of mesenchymal stem cells even with endothelial growth medium. Biotechnol Lett 33, 2351–2359 (2011). https://doi.org/10.1007/s10529-011-0706-5
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DOI: https://doi.org/10.1007/s10529-011-0706-5