Abstract
Lipase Lip2 from Yarrowia lipolytica was displayed on the cell surface of Saccharomyces cerevisiae using Cwp2 as an anchor protein. Successful display of the lipase on the cell surface was confirmed by immunofluorescence microscopy and halo assay. The length of linker sequences was further examined to confirm that the correct conformation of Lip2 was maintained. The results showed that the displayed Lip2 exhibited the highest activity at 7.6 ± 0.4 U/g (dry cell) when using (G4S)3 sequence as the linker, with an optimal temperature and pH at 40°C and pH 8.0. The displayed lipase did not lose any activity after being treated with 0.1% Triton X-100 and 0.1% Tween 80 for 30 min, and it retained 92% of its original activity after incubation in 10% DMSO for 30 min. It also exhibited better thermostability than free Lip2 as reported previously.
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Acknowledgements
The authors are grateful for kind present of plasmids from Dr. Frank Breinig and Dr. Manfred J. Schmitt from Angewandte Molekularbiologie, Universitat des Saarlandes, D-66041 Saarbrucken, Germany. This work was funded by National High Technology Research and Development Program of China (863 Program) (2006AA020203, 2007AA05Z417, 2007AA100703, 2009AA03Z232), Program for New Century Excellent Talents in University (NCET-07-0336), and Wuhan Project of Science and Technology (200720422138).
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Liu, W., Zhao, H., Jia, B. et al. Surface display of active lipase in Saccharomyces cerevisiae using Cwp2 as an anchor protein. Biotechnol Lett 32, 255–260 (2010). https://doi.org/10.1007/s10529-009-0138-7
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DOI: https://doi.org/10.1007/s10529-009-0138-7