Abstract
Lentivirus(LV)-mediated small interfering RNA (siRNA) targeting tumor necrosis factor-α (TNF-α) was locally administered into the air pouch of mice to inhibit inflammation induced by titanium alloy particles. The lentiviral vector expressed green fluorescent protein (GFP) as a reporter gene. Down-regulation of TNF-α in pouch area was confirmed by real-time PCR and ELISA, resulting in significantly decreased local inflammatory responses (P < 0.01). This approach was proven safe by localized GFP fluorescence and invariant TNF-α expression in peripheral blood, liver, spleen, kidney, lung and brain of mouse. In conclusion, locally administered siRNA provides an effective and safe method for inhibiting particle-induced inflammation.
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Acknowledgements
This work was supported by PhD Program of Shanghai Jiaotong University, 2007. Hereby, we would like to express our great appreciation to the following people from Medical College of Shanghai Jiaotong University for their kindness and support in this study: Chao Zhang, Xiaoxiao Zhou, Wen Wu, Kun Tao, Tao Cheng, Jia Jiang, Feng Wang.
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Peng, X., Zhang, X. & Zeng, B. Locally administered lentivirus-mediated siRNA inhibits wear debris-induced inflammation in murine air pouch model. Biotechnol Lett 30, 1923–1929 (2008). https://doi.org/10.1007/s10529-008-9788-0
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DOI: https://doi.org/10.1007/s10529-008-9788-0