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Permeabilization and lysis induced by bacteriocins and its effect on aldehyde formation by Lactococcus lactis

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Abstract

Permeabilization induced by lacticin 3147, lactococcins A, B and M, enterocin AS-48 and nisin, bacteriocins described as cell membrane-pore forming and lytic agents, enhanced in all cases aldehyde formation by Lactococcus lactis IFPL730. Nevertheless, the conversion of isoleucine into 2-methylbutyraldehyde depended not only on the degree of permeabilization but also on the bacteriocin that caused the cell membrane damage. The highest values of 2-methylbutyraldehyde corresponded to cell suspensions containing lacticin 3147 and lactococcins, treatments that provoked further lysis in addition to induced permeabilization.

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Acknowledgements

This work was supported by the Research Project CICYT AGL2002-03277. The authors also would like to thank Prof. Dr. Jan Kok and Dr.␣Margarita Medina for kindly providing microorganisms L. lactis subsp. cremoris B94 and Enterococcus faecalis INIA4, respectively, and Pedro Lastres for their assistance with the Flow Cytometry analyses.

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  1. Department of Dairy Science and Technology, Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria, 28040, Madrid, Spain

    M. Carmen Martínez-Cuesta, Teresa Requena & Carmen Peláez

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  1. M. Carmen Martínez-Cuesta
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  2. Teresa Requena
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  3. Carmen Peláez
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Correspondence to M. Carmen Martínez-Cuesta.

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Martínez-Cuesta, M.C., Requena, T. & Peláez, C. Permeabilization and lysis induced by bacteriocins and its effect on aldehyde formation by Lactococcus lactis . Biotechnol Lett 28, 1573–1580 (2006). https://doi.org/10.1007/s10529-006-9131-6

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  • DOI: https://doi.org/10.1007/s10529-006-9131-6

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