Abstract
By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l−1) than when grown in the unsupplemented medium.
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Acknowledgements
We like to thank Dr. Wong for kind provision of strain WB700 and plasmid pWB980. Special thanks also go to reviewers for their valuable comments.
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Chen, P.T., Chao, YP. Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors. Biotechnol Lett 28, 1595–1600 (2006). https://doi.org/10.1007/s10529-006-9126-3
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DOI: https://doi.org/10.1007/s10529-006-9126-3