Abstract
Protein transduction domain (PTD)-mediated protein delivery into animal cells is a useful technique for regulating cellular functions. Proteins captured by antibodies were delivered into living cells using an antibody/PTD-fused protein A complex. As a model protein, fluorescent-modified antibodies, captured by their respective primary antibody, were analyzed by fluorescence-activated cell sorting (FACS) which showed that the fluorescent-modified antibodies were directly delivered into cells. Peroxidase, captured by its specific antibody, was also delivered into cells and retained its activity.
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Acknowledgement
This work was supported in part by Core Research for Evolutional Science and Technology (CREST) from Japan Science and Technology Agency (JST) and Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (JSPS).
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Mie, M., Mori, K., Funabashi, H. et al. Delivery of antibody-captured proteins into living cells using PTD-fused protein A. Biotechnol Lett 28, 1209–1214 (2006). https://doi.org/10.1007/s10529-006-9076-9
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DOI: https://doi.org/10.1007/s10529-006-9076-9