Skip to main content
Log in

Induction of Glutathione S-Transferase and Lutathione by Toxic Compounds and Elicitors in Reed Canary Grass

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Treatment of read canary grass leaves with phenol, 4-chlorophenol, naphthalic anhydride and phenylethylisothiocyanate increased glutathione S-transferase activity by 1.4–2.4-fold (control 17 U g−1 DW). Benzothiadiazole, β-aminobutyric acid and salicylic acid increased activity by 1.3–1.8-fold. Total glutathione pool was increased by the toxic compounds by 1.2–2-fold and by the elicitors 1.4–1.6-fold (control 593 nmol  g−1 DW). Unlike the other compounds, benzothiadiazole and salicylic acid did not decrease the redox state. Benzothiadiazole acted synergistically with chlorophenol on glutathione S-transferase and glutathione levels and counteracted the decrease in redox state caused by the xenobiotic. Reed canary grass thus has a strong potential to neutralize toxic compounds, which may be further enhanced by elicitors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Q Chaudhry P Schröder D Werck-Reichhart W Grajek R Marecik (2002) ArticleTitleProspects and limitations of phytoremediadion for the removal of persistent pesticides in the environment Environ. Sci. Pollut. Res 9 4–17

    Google Scholar 

  • JOD Coleman MMA Blake-Kalff TG Emyr Davies (1997) ArticleTitleDetoxification of xenobiotics by plants: chemical modification and vacuolar compartmentation Trends. Plant Sci 2 144–151 Occurrence Handle10.1016/S1360-1385(97)01019-4

    Article  Google Scholar 

  • R Edwards DP Dixon (2004) ArticleTitleMetabolism of natural and xenobiotic substrates by the plant glutathione S-transferase superfamily Ecological Studies 170 17–50

    Google Scholar 

  • JW Fahey AT Zalcmann P Talalay (2001) ArticleTitleThe chemical diversity and distribution of glucosinolates and isothiocyanates among plants Phytochemistry 56 5–51 Occurrence Handle10.1016/S0031-9422(00)00316-2 Occurrence Handle11198818

    Article  PubMed  Google Scholar 

  • OW Griffith (1980) ArticleTitleDetermination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine Anal. Biochem 106 207–212 Occurrence Handle10.1016/0003-2697(80)90139-6 Occurrence Handle7416462

    Article  PubMed  Google Scholar 

  • OC Knörzer B Lederer J Durner P Böger (1999) ArticleTitleAntioxidative defense activation in soybean cells Physiol. Plant 107 294–302 Occurrence Handle10.1034/j.1399-3054.1999.100306.x

    Article  Google Scholar 

  • F Navari-Izzo R Izzo (1994) ArticleTitleInduction of enzyme activities and antioxidant production in barley plants as a result of SO2 fumigation Plant Sci 96 31–40 Occurrence Handle10.1016/0168-9452(94)90219-4

    Article  Google Scholar 

  • M Oostendorp W Kunz B Dietrich T Staub (2001) ArticleTitleInduced disease resistance in plants by chemicals Eur. J. Plant Pathol 107 19–28 Occurrence Handle10.1023/A:1008760518772

    Article  Google Scholar 

  • S Pascal R Scalla (1999) ArticleTitlePurification and characterization of a safener-induced glutathione S-transferase from wheat (Triticum aestivum) Physiol. Plant 106 17–27 Occurrence Handle10.1034/j.1399-3054.1999.106103.x

    Article  Google Scholar 

  • A Schäffner B Messner C Langebartels H Sandermann (2002) ArticleTitleGenes and enzymes for in-planta phytoremediation of air, water and soil Acta Biotechnol 22 141–152 Occurrence Handle10.1002/1521-3846(200205)22:1/2<141::AID-ABIO141>3.0.CO;2-7

    Article  Google Scholar 

  • P Schröder C Collins (2002) ArticleTitleConjugating Enzymes involved in xenobiotic metabolism of organic xenobiotics in plants Int. J. Phytoremed 4 247–265

    Google Scholar 

  • T Tausz H Šircelj D Grill (2004) ArticleTitleThe glutathione system as a stress marker in plant ecophysiology: is a stress–response concept valid? J. Exp. Bot 55 1955–1962 Occurrence Handle10.1093/jxb/erh194 Occurrence Handle15234995

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to H. Urbanek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Urbanek, H., Majorowicz, H., Zalewski, M. et al. Induction of Glutathione S-Transferase and Lutathione by Toxic Compounds and Elicitors in Reed Canary Grass. Biotechnol Lett 27, 911–914 (2005). https://doi.org/10.1007/s10529-005-7181-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-7181-9

Keywords

Navigation