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Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

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Abstract

The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-β-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg−1.

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Acknowledgments

The authors gratefully acknowledge to Profs. Rajni Hatti-Kaul and Bo Mattiasson. This work was supported by CONICET, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina) and by the Swedish International Development Cooperation Agency (SIDA).

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Authors and Affiliations

  1. PROIMI, Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, T-400 1MVB, Tucumán, Argentina

    M. Alejandra Martínez, Mario D. Baigorí & Faustino Siñeriz

  2. Department of Biotechnology, Centre for Chemistry & Chemical Engineering, Lund University, P.O. Box 124, , SE-221 00, Lund, Sweden

    M. Alejandra Martínez & Osvaldo D. Delgado

Authors
  1. M. Alejandra Martínez
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  2. Osvaldo D. Delgado
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  3. Mario D. Baigorí
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  4. Faustino Siñeriz
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Correspondence to M. Alejandra Martínez.

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Martínez, M.A., Delgado, O.D., Baigorí, M.D. et al. Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans. Biotechnol Lett 27, 545–550 (2005). https://doi.org/10.1007/s10529-005-2879-2

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  • DOI: https://doi.org/10.1007/s10529-005-2879-2

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