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Hydroxynitrile Lyase Catalysis in Ionic Liquid-containing Systems

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Abstract

The cleavage of mandelonitrile catalysed by hydroxynitrile lyases (HNL) from Prunus amygdalus (PaHNL) and Manihot esculenta (MeHNL) proceeded more rapidly in monophasic aqueous media containing 1-propyl-3-methylimidazolium tetrafluoroborate [C4MIm][BF4] than in media containing acetonitrile or THF. Both HNLs were much more thermostable in [C4MIm][BF4] than in acetonitrile or THF. The addition of each of the four ionic liquids 1-butyl-, 1-pentyl- and 1-hexyl-3-methylimidazolium tetrafluoroborates at 2–6% (v/v in the aqueous phase) increased both the enzyme activity and the product e.e. in the PaHNL-catalysed transcyanation in an aqueous/DIPE biphasic system. However, MeHNL was inactivated by the ionic liquids, as indicated by the decreased reaction rate, substrate conversion and product e.e.

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Authors and Affiliations

  1. Department of Biotechnology, South China University of Technology, 510640, Guangzhou, P.R. China

    Wen-Yong Lou, Ruo Xu & Min-Hua Zong

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  1. Wen-Yong Lou
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  2. Ruo Xu
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  3. Min-Hua Zong
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Correspondence to Min-Hua Zong.

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Lou, WY., Xu, R. & Zong, MH. Hydroxynitrile Lyase Catalysis in Ionic Liquid-containing Systems. Biotechnol Lett 27, 1387–1390 (2005). https://doi.org/10.1007/s10529-005-0686-4

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  • DOI: https://doi.org/10.1007/s10529-005-0686-4

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