Abstract
Glucocorticoid-induced cataract (GIC)-associated biomarkers were screened by ceRNA network construction. The GIC samples’ GSE3040 were obtained from the NCBI-GEO database. R’s Limma package was used to identify differentially expressed RNAs (DERs) between the normal and GIC samples group (4- and 16-h). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis for the mRNAs in the constructed GIC lncRNA–miRNA–mRNA ceRNA regulation network was implemented. A total of 1665 and 1443 DERs were obtained in the 4- and 16-h group, respectively. At two time points, 256 overlapping DERs were identified, of which 210 (17 lncRNAs and 203 mRNAs) had significant differential expression (4 down- and 206 up-regulated). A total of 534 co-expressed ligation pairs (all up-regulated) were obtained. A ceRNA regulation network was constructed. RPS6KA5, GAB1, CCR7, CCL2, COL4A4, and PPARG were obtained and significantly enriched in the 4 KEGG signaling pathways and were featured as GIC target molecules.
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Availability of Data and Material
The datasets generated during and/or analyzed during the current study are available in the [GEO] repository, [https://www.ncbi.nlm.nih.gov/)].
Abbreviations
- GIC:
-
Glucocorticoid-induced cataract
- DERs:
-
Differentially expressed RNAs
- PCC:
-
Pearson correlation coefficient
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- ncRNAs:
-
Non-coding RNAs
- lncRNAs:
-
Long non-coding RNAs
- DEX:
-
Dexamethasone
- HGNC:
-
HUGO Gene Nomenclature Committee
- GO:
-
Gene Ontology
- ceRNA:
-
Competitive endogenous RNA
- GC:
-
Glucocorticoids
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This work was supported by the Heilongjiang Province Postdoctoral Funds (grant number LBH-Z18194).
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PL and ZS were responsible for the conception and design of the research, and drafting the manuscript. X-M Z and H-Y G performed the data acquisition. Y S and CW performed the data analysis and interpretation. ZS and H-YG participated in the design of the study and performed the statistical analysis. All authors read and approved the final manuscript.
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Shi, Z., Zhao, X., Su, Y. et al. Screening of Biological Target Molecules Related to Glucocorticoid-Induced Cataract (GIC) on the Basis of Constructing ceRNA Network. Biochem Genet 60, 24–38 (2022). https://doi.org/10.1007/s10528-021-10078-3
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DOI: https://doi.org/10.1007/s10528-021-10078-3