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Development of Genomic SSR Markers and Molecular Characterization of Magnaporthe oryzae Isolates from Wheat in Brazil

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Abstract

Magnaporthe oryzae, the causal agent of wheat blast, was characterized on a molecular level with 38 newly isolated genomic SSR loci. Among the 31 wheat isolates analyzed, 15 polymorphic loci were detected, with an average of 1.7 alleles per locus, 28.9% of them being highly or reasonably informative. The number of polymorphic loci was higher in isolates from Londrina in the Brazilian state of Paraná and Coromandel in Minas Gerais compared with Goiânia in Goiás and São Borja in Rio Grande do Sul. The rice isolate was clearly different from the wheat isolates, and the size difference in polymorphic SSR loci between one isolate from wheat and one isolate from rice was associated with the number of repeats. Some isolates collected from different states and in different years demonstrated similarities of 100%. The markers developed here are useful for the genetic analysis of M. oryzae isolated from wheat, and isolates representing the variability detected in the field can be used to search for better wheat blast resistance.

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Acknowledgments

This research was supported by the project entitled “Desenvolvimento e uso de marcadores de regiões hipervariáveis do genoma de Magnaporthe grisea do trigo para análise de isolados do fungo com virulência caracterizada” (project SEG code number 03.06.05.014.00.00 Agrofuturo).

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Authors and Affiliations

  1. Embrapa Trigo, Passo Fundo, RS, 99001-970, Brazil

    Jorge Fernando Pereira, Luciano Consoli, Ana Lídia Variani Bonato & João Leodato Nunes Maciel

  2. Universidade Luterana do Brasil, Carazinho, RS, 99500-000, Brazil

    Estevon Alexandre de Souza Bombonatto

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  1. Jorge Fernando Pereira
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  2. Luciano Consoli
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  3. Estevon Alexandre de Souza Bombonatto
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  4. Ana Lídia Variani Bonato
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  5. João Leodato Nunes Maciel
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Correspondence to João Leodato Nunes Maciel.

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Pereira, J.F., Consoli, L., de Souza Bombonatto, E.A. et al. Development of Genomic SSR Markers and Molecular Characterization of Magnaporthe oryzae Isolates from Wheat in Brazil. Biochem Genet 52, 52–70 (2014). https://doi.org/10.1007/s10528-013-9627-4

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