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DNA Sequence Variation of the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Determined by Mitochondrial A+T-rich Region and Nuclear ITS2 Sequences

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Abstract

In an effort to gain greater insight into the nature of the population genetic structure of the pest insect Spodoptera litura (Lepidoptera: Noctuidae), tobacco cutworms were collected from six Korean and five Chinese localities, and their mitochondrial A+T-rich region and nuclear internal transcribed spacer 2 (ITS2) were cloned and sequenced. The A+T-rich region and nuclear ITS2 provided a maximum sequence divergence of 2.88 and 1.82%, respectively. Overall, a low level of genetic fixation (F ST = 0–0.02965 in the A+T-rich region and 0–0.34491 in ITS2) and no discernible isolated population were noted among most S. litura populations. Along with these results, the absence of genetic variance between Korea and China indicates a profound interrelation of the S. litura populations in the two countries, consistent with the current notion that S. litura has sufficient flight capacity for dispersal.

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Acknowledgment

This study was supported by a grant from the Agenda Program (no. 200901FHT020609413) from the Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Xinlong Wan, Min Jee Kim & Iksoo Kim

  2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Jianhong Li

  3. Department of Agricultural Biology, National Academy of Agricultural Science, Suwon, 441-100, Republic of Korea

    Hae Chul Park

  4. Research Institute for East Asian Environment and Biology, Seoul, 293-27, Republic of Korea

    Sung-Soo Kim

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  1. Xinlong Wan
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Correspondence to Iksoo Kim.

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Wan, X., Li, J., Kim, M.J. et al. DNA Sequence Variation of the Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae), Determined by Mitochondrial A+T-rich Region and Nuclear ITS2 Sequences. Biochem Genet 49, 760–787 (2011). https://doi.org/10.1007/s10528-011-9449-1

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