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Detection of Bovine Spongiform Encephalopathy-Related Prion Protein Gene Promoter Polymorphisms in Local Turkish Cattle

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Abstract

Polymorphisms in open reading frames of the prion protein gene (PRNP) have been shown to be associated with prion disease susceptibility in humans, sheep, and mice. Studies in recent years have demonstrated a similar effect of PRNP promoter and intron-1 polymorphisms on bovine spongiform encephalopathy (BSE) susceptibility in cattle. In this study, the deletion/insertion (indel) polymorphisms of the bovine PRNP gene within the promoter sequence (23 bp) and intron 1 (12 bp) were analyzed in local Turkish cattle. For this, 150 animals belonging to three different local breeds—the South Anatolian red, the East Anatolian red, and the Turkish gray—were tested using DNA purification and polymerase chain reaction. The ins allele in the 12 bp indel, which is associated with low susceptibility to BSE, showed a high frequency in all three breeds. The low-susceptibility allele of the 23-bp indel was identified in Turkish gray cattle with a frequency of 0.80. Results of the study have shown that local Turkish cattle might have an important genetic value for selection against BSE.

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Acknowledgments

This work was supported by the Research Fund of the University of Istanbul, Project No. UDP-1971/18012008. The authors would like to thank the Turkish Scientific Council (TUBİTAK) and the German Scientific Council (DFG), which supported the stay of Dr. Cemal Ün for three months at the University of Bonn, Institute of Animal Sciences.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences and Arts, Yıldız Technical University, Esenler, 34210, Merter Istanbul, Turkey

    Cemal Ün & Nehir Özdemir

  2. Department of Biochemistry, Faculty of Veterinary Medicine, University of Istanbul, Avcılar, Istanbul, Turkey

    Kemal Oztabak & Ahmet Mengi

  3. Department of Animal Breeding, Institute of Animal Sciences, University of Bonn, Endenicher Allee 15, 53115, Bonn, Germany

    Dawit Tesfaye & Karl Schellander

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  1. Cemal Ün
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  2. Kemal Oztabak
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  3. Nehir Özdemir
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  4. Dawit Tesfaye
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  5. Ahmet Mengi
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  6. Karl Schellander
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Correspondence to Cemal Ün.

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Ün, C., Oztabak, K., Özdemir, N. et al. Detection of Bovine Spongiform Encephalopathy-Related Prion Protein Gene Promoter Polymorphisms in Local Turkish Cattle. Biochem Genet 46, 820–827 (2008). https://doi.org/10.1007/s10528-008-9196-0

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  • DOI: https://doi.org/10.1007/s10528-008-9196-0

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