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Chryseobacterium nankingense sp. nov. WR21 effectively suppresses Ralstonia solanacearum growth via intensive root exudates competition

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Abstract

Previous studies demonstrated that the Chryseobacterium sp. WR21 could effectively control the bacterial wilt disease caused by Ralstonia solanacearum through effective root colonization. The strain WR21 exhibited a low level of DNA homology with Chryseobacterium strains DSM 15235T (24.1%), DSM 17724T (24.8%), and DSM 18014T (10.4%), suggesting that WR21 may represent a novel species, for which the name Chryseobacterium nankingense sp. nov. is proposed. The in vitro competition experiments with strain WR21 indicated it significantly inhibited growth of the pathogen in co-culture with six of nine tested nutrients (e.g. root exudates) that could be utilized by strain WR21 and R. solanacearum. Similar trends were observed in co-culturing experiments using tissue exudates of tomato. A positive relationship (r = 0.785) was noticed between the differences in the average growth rate of both strains and the disease suppression effects. In conclusion, Chryseobacterium nankingense sp. nov. WR21 exhibits antagonism through nutrient competition that might be used for achieving biocontrol of Ralstonia solanacearum induced wilts.

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Acknowledgements

We thank Prof. Shixue Yin from Yangzhou University and Dr. Jun Zhang from Nanjing Agricultural University for helpful assistance in the identification of Chryseobacterium nankingense strain WR21. We also are highly grateful to Prof. Dr. mark L Gleason for the correction of this manuscript. This research was financially supported by the National Natural Science Foundation of China (31501837 to Jianfeng Huang, 41471213 to Yangchun Xu, 41671248 and 41301262 to Zhong Wei), the National Key Basic Research Program of China (2015CB150503 to Qirong Shen), the Natural Science Foundation of Jiangsu Province (BK20130677 to Zhong Wei), the China Postdoctoral Science Foundation (2013M541687), the Young Elite Scientist Sponsorship Program by CAST (2015QNRC001 to Zhong Wei), and the Qing Lan Project (funding to Yangchun Xu and Zhong Wei).

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Authors and Affiliations

  1. Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Jianfeng Huang, Zhong Wei, Jie Hu, Chunlan Yang, Yi’an Gu, Xinlan Mei, Qirong Shen & Yangchun Xu

  2. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation/Key Laboratory of Plant Nutrition and Fertilizer in South Region, Guangzhou, 510640, People’s Republic of China

    Jianfeng Huang

  3. United States Pakistan Center for Advanced Studies in Agriculture and Food Security (USPCAS-AFS)/Microbiomes and Genomics Laboratory, Department of Plant Pathology, University of Agriculture Faisalabad, Faisalabad, 38000, Pakistan

    Kashif Riaz

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  1. Jianfeng Huang
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  2. Zhong Wei
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  3. Jie Hu
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Correspondence to Yangchun Xu.

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Huang, J., Wei, Z., Hu, J. et al. Chryseobacterium nankingense sp. nov. WR21 effectively suppresses Ralstonia solanacearum growth via intensive root exudates competition. BioControl 62, 567–577 (2017). https://doi.org/10.1007/s10526-017-9812-1

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