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Biocontrol of Fusarium wilt disease in muskmelon with Bacillus subtilis Y-IVI

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Abstract

Muskmelon (Cucumis melo L.) wilt caused by Fusarium oxysporum f. sp. melonis leads to severe economic losses. A bio-organic fertilizer (BIO) fortified with an antagonistic strain of Bacillus subtilis Y-IVI was used to control this disease. Pot experiments were carried out to investigate the efficacy and to elucidate biocontrol mechanisms for the disease. BIO significantly reduced the disease incidence. Population of F. oxysporum in plant shoots of the BIO treatment were about 1000-fold lower than the control. Population of Y-IVI remained high in muskmelon rhizosphere of the BIO treatment during the experiment. Concentration of antifungal lipopeptides, iturin A, in the BIO treatment was significantly higher than other treatments. Ten days after transplantation, the salicylic acid content in BIO-treated plant leaves was significantly higher than control. In conclusion, BIO effectively controlled muskmelon wilt, possibly because the antagonistic microbes effectively colonize the plant rhizosphere and shoots to preclude pathogen invasion. Furthermore, Y-IVI produces antifungal lipopeptides in the rhizosphere.

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Acknowledgments

This work was supported by the China Science and Technology Ministry project “Basic Research on Fertilizer Saving and Efficiency Improvement for Sustainable Utilization of Farmland” (No. 2007CB109304), Jiangsu Provincial Key Technology R&D Program (No. BE2009672) and Suzhou Science and Technology Program (No. SNG201023). We would like to thank Dr. A. J. Miller from Rothamsted Research Station, UK, for revising this manuscript.

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Authors and Affiliations

  1. Jiangsu Provincial Key Lab of Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China

    Qingyun Zhao, Wei Ran, Xiang Li, Qirong Shen & Yangchun Xu

  2. Spice and Beverage Research Institute, China Academy of Tropical Agricultural Sciences, Haikou, 571533, China

    Qingyun Zhao & Hui Wang

  3. Wujiang Agriculture and Technology Generalize Center, Wujiang, 215200, China

    Shengyuan Shen

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  1. Qingyun Zhao
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  6. Shengyuan Shen
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  7. Yangchun Xu
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Correspondence to Yangchun Xu.

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Handling Editor: Choong-Min Ryu

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Zhao, Q., Ran, W., Wang, H. et al. Biocontrol of Fusarium wilt disease in muskmelon with Bacillus subtilis Y-IVI. BioControl 58, 283–292 (2013). https://doi.org/10.1007/s10526-012-9496-5

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  • DOI: https://doi.org/10.1007/s10526-012-9496-5

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