Abstract
Understanding food web interactions in native or agricultural ecosystems is an important step towards establishing sustainable pest management strategies. While the role of generalist predators as biological control agents is increasingly appreciated, the study of trophic interactions between individual predator species and their prey provides practical difficulties. Recently, different approaches have been suggested to determine prey items from predator guts using molecular methods. Macrolophus caliginosus is a generalist predator active in herbaceous agro-ecosystems. We developed a system to identify the DNA of its prey after ingestion, using Myzus persicae as a model. Esterase (MpEST) and cytochrome oxidase I (MpCOI) genes were targeted in the aphid, while M. caliginosus COI gene was used as control for predator DNA. Real time PCR proved to be specific and sensitive enough to detect prey DNA upon ingestion after feeding experiments. The system provided a linear amplification response with only 10 fg of prey genomic DNA as template. The detection system of MpCOI gene was more sensitive than MpEST, while the detection period was similar for both genes. Possibilities for using the system in ecological and biosafety studies with regard to sustainable pest management are discussed.
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Acknowledgments
The authors thank Grazia Maria Di Leo for maintaining the aphid colony and Fabienne Morgues for help in molecular validations. This study was supported by the ENEA External Relations Annual Research Fellowship Programme to JEUS and JRMA.
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Genbank accession numbers: M. caliginosus COI: AY855089.
Handling Editor: Eric Lucas.
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Schmidt, J.E.U., Almeida, J.R.M., Rosati, C. et al. Identification of trophic interactions between Macrolophus caliginosus (Heteroptera: Miridae) and Myzus persicae (Homoptera: Aphididae) using real time PCR. BioControl 54, 383–391 (2009). https://doi.org/10.1007/s10526-008-9194-5
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DOI: https://doi.org/10.1007/s10526-008-9194-5