A promising approach to the development of new means for preventing infection caused by tick-borne encephalitis virus can be DNA vaccines encoding polyepitope T-cell immunogens. A DNA vaccine pVAX-AG4-ub encoding an artificial polyepitope immunogen that includes cytotoxic and T-helper epitopes from the NS1, NS3, NS5, and E proteins of the tick-borne encephalitis virus has been obtained. The developed construct ensured the synthesis of the corresponding mRNAs in transfected eukaryotic cells. Immunization of mice with pVAX-AG4-ub induced the formation of a virus-specific T-cell response providing 50% protection from lethal infection with the virus.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 176, No. 7, pp. 85-89, July, 2023
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Kisakov, D.N., Antonets, D.V., Shaburova, E.V. et al. DNA Vaccine Encoding the Artificial T-Cell Polyepitope Immunogen of Tick-Borne Encephalitis Virus. Bull Exp Biol Med 176, 72–76 (2023). https://doi.org/10.1007/s10517-023-05970-4
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DOI: https://doi.org/10.1007/s10517-023-05970-4