Short linear peptide fragments of placental trophoblastic β1-glycoprotein (PSG) (YECE, YQCE, YVCS, and YACS) were studied in the context of their immunomodulatory effects at the level of inflammatory markers. The original host-versus-graft model was used in male Wistar rats without prior conditioning of recipient bone marrow. A composition of PSG peptide fragments was injected to animals after allogeneic transplantation of bone marrow cells in a dynamic experiment, inflammatory markers α1-acid glycoprotein (AGP, orosomucoid), α2-macroglobulin (α2M) were assayed by ELISA, and biochemical parameters (total protein, glucose, creatinine, and urea) were measured. The levels of α2M and AGP increased in response to allotransplantation, whereas administration of PSG peptides normalized serum α2M levels by the end of the experiment. The decrease in α2M level coincided with the independent effect of PSG peptide administration. The levels of total protein, glucose, creatinine, and urea in rat serum after allotransplantation were reduced throughout the experiment. Administration of PSG peptides contributed to normalization of serum total protein, creatinine, and urea levels by the end of the experiment. Administration of PSG peptides after allogeneic transplantation of bone marrow suspension contributed to normalization of the levels of α2M, total protein, creatinine, and urea, which can be interpreted as an anti-inflammatory effect of these peptides.
Similar content being viewed by others
References
Apostolopoulos V, Bojarska J, Chai TT, Elnagdy S, Kaczmarek K, Matsoukas J, New R, Parang K, Lopez OP, Parhiz H, Perera CO, Pickholz M, Remko M, Saviano M, Skwarczynski M, Tang Y, Wolf WM, Yoshiya T, Zabrocki J, Zielenkiewicz P, AlKhazindar M, Barriga V, Kelaidonis K, Sarasia EM, Toth I. A global review on short peptides: frontiers and perspectives. Molecules. 2021;26(2):430. doi: https://doi.org/10.3390/molecules26020430
Wang L, Wang N, Zhang W, Cheng X, Yan Z, Shao G, Wang X, Wang R, Fu C. Therapeutic peptides: current applications and future directions. Signal Transduct. Target Ther. 2022;7(1):48. doi: https://doi.org/10.1038/s41392-022-00904-4
Moldogazieva NT, Mokhosoev IM, Terentiev AA. Pregnancy-specific β1-glycoproteins: combined biomarker roles, structure/function relationships and implications for drug design. Curr. Med. Chem. 2017;24(3):245-267. doi: https://doi.org/10.2174/0929867324666161123090554
Reagan WJ, Shoieb AM, Schomaker SJ, Markiewicz VR, Clarke DW, Sellers RS. Evaluation of rat acute phase proteins as inflammatory biomarkers for vaccine nonclinical safety studies. Toxicol. Pathol. 2020;48(7):845-856. doi: https://doi.org/10.1177/0192623320957281
Serrano I, Luque A, Aran JM. Exploring the immunomodulatory moonlighting activities of acute phase proteins for tolerogenic dendritic cell generation. Front. Immunol. 2018;9:892. doi: https://doi.org/10.3389/fimmu.2018.00892
Washington IM, Van Hoosier G. Clinical Biochemistry and Hematology. The Laboratory rabbit, guinea pig, hamster, and other rodents. Suckow M, Stevens K, Wilson R, eds. Academic Press, 2011. P. 57-116.
Schroeder MA, DiPersio JF. Mouse models of graft-versus-host disease: advances and limitations. Dis. Model Mech. 2011;4(3):318-333. doi: https://doi.org/10.1242/dmm.006668
Boieri M, Shah P, Dressel R, Inngjerdingen M. The role of animal models in the study of hematopoietic stem cell transplantation and GvHD: a historical overview. Front. Immunol. 2016;7:333. doi: https://doi.org/10.3389/fimmu.2016.00333
Hong T, Li SM, Huang Y, Shu K, Li LX, Liu L, Liu ZY. Evaluation of the Cytotoxicity and immune and subacute toxicity of camptothecin-loaded nanoparticles. Pharm. Anal Acta. 2019;10(1):604. doi: https://doi.org/10.35248/2153-2435.19.10.604
Rezvani AR, Storer BE, Storb RF, Mielcarek M, Maloney DG, Sandmaier BM, Martin PJ, McDonald GB. Decreased serum albumin as a biomarker for severe acute graft-versus-host disease after reduced-intensity allogeneic hematopoietic cell transplantation. Biol. Blood Marrow Transplant. 2011;17(11):1594-1601. doi: https://doi.org/10.1016/j.bbmt.2011.07.021
Cray C, Zaias J, Altman NH. Acute phase response in animals: a review. Comp. Med. 2009;59(6):517-526.
Bode JG, Albrecht U, Häussinger D, Heinrich PC, Schaper F. Hepatic acute phase proteins--regulation by IL-6- and IL-1-type cytokines involving STAT3 and its crosstalk with NF-κB-dependent signaling. Eur. J. Cell Biol. 2012;91(6-7):496-505. doi: https://doi.org/10.1016/j.ejcb.2011.09.008
Timganova VP, Bochkova MS, Shardina KYu, Uzhviyuk SV, Gutina EV, Rayev MB, Lyubimov AV, Zamorina SA. Effect of short PSG peptide fragments on the cytokine profile in Wistar rats during allogeneic transplantation in vivo. Med. Immunol. 2022;24(3):491-506. Russian. doi: https://doi.org/10.15789/1563-0625-EOS-2472
Zaias J, Mineau M, Cray C, Yoon D, Altman NH. Reference values for serum proteins of common laboratory rodent strains. J. Am. Assoc. Lab Anim. Sci. 2009;48(4):387-390.
Soeters PB, Wolfe RR, Shenkin A. Hypoalbuminemia: pathogenesis and clinical significance. JPEN J. Parenter. Enteral Nutr. 2019;43(2):181-193. doi: https://doi.org/10.1002/jpen.1451
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 5, pp. 591-596, May, 2023
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Bochkova, M.S., Timganova, V.P., Uzhviyuk, S.V. et al. Effect of Short PSG Peptides on Inflammatory Markers in Allogeneic Bone Marrow Cell Transplantation in Wistar Rats. Bull Exp Biol Med 175, 653–657 (2023). https://doi.org/10.1007/s10517-023-05920-0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-023-05920-0