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Genotyping of Macaque Population on the Brain-Derived Neurotrophic Factor Gene Polymorphism by Mismatch Amplification Mutation Assay (MAMA)-PCR

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Bulletin of Experimental Biology and Medicine Aims and scope

Human rs6265 (196G>A) polymorphism in the BDNF gene is associated with many clinically significant phenotypic manifestations. Rhesus monkey (Macaca mulatta) has a functionally significant rs309950446 ( 136G>A) polymorphism. To determine this polymorphism in macaques, we used mismatch amplification mutation assay (MAMA)-PCR method with non-complementary nucleotide to the template chain at the 3rd position from the 3’-end of the allele-specific primers (mismatch primers), which allowed the best discrimination of the alleles. Genotyping of male rhesus monkeys (n=178) and cynomolgus monkeys (Macaca fascicularis) (n=90) was carried out. The A/A, G/G, and G/A genotypes were found in 16, 34, and 50% rhesus macaques, respectively. In the cynomolgus macaques, the mutant polymorphic allele was not detected. The study results allow considering rhesus macaques as a potential biological model for assessment of the gen—environment interaction of the BDNF gene polymorphism.

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Authors and Affiliations

  1. Research Institute of Medical Primatology, Sochi, Russia

    L. E. Pavlova, M. F. Timina, A. A. Agumava & A. V. Panchenko

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  1. L. E. Pavlova
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  2. M. F. Timina
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  3. A. A. Agumava
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  4. A. V. Panchenko
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Correspondence to L. E. Pavlova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 175, No. 3, pp. 374-378, March, 2023

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Pavlova, L.E., Timina, M.F., Agumava, A.A. et al. Genotyping of Macaque Population on the Brain-Derived Neurotrophic Factor Gene Polymorphism by Mismatch Amplification Mutation Assay (MAMA)-PCR. Bull Exp Biol Med 175, 388–392 (2023). https://doi.org/10.1007/s10517-023-05873-4

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  • DOI: https://doi.org/10.1007/s10517-023-05873-4

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